Biosecurity Sciences Laboratory, Biosecurity Queensland, Department of Agriculture and Fisheries, Queensland Government, Health and Food Sciences Precinct, 39 Coopers Plains, 4108, Queensland, Australia.
Berrimah Veterinary Laboratory, Department of Industry, Tourism and Trade, Berrimah, 0801, Northern Territory, Australia.
J Gen Virol. 2021 Dec;102(12). doi: 10.1099/jgv.0.001685.
Middle Point orbivirus (MPOV) is an Australian arbovirus, belongs to the species found in China. First detected and reported from Beatrice Hill, Northern Territory (NT), MPOV has to date, only been exclusively reported from the NT, Australia. Whilst genetic characterization of MPOV has been previously described, only restricted to sequence information for segments 2 and 3 coding core protein VP2 and outer capsid protein VP3, respectively. This study presents for the first time nearly full-length genome sequences of MPOV, which represent 24 isolates collected over a span of more than 20 years from 1997 to 2018. Whilst the majority of isolates were sampled at Beatrice Hill, NT where MPOV is most frequently isolated, this report also describes the first two isolations of MPOV from Queensland (QLD), Australia. One of which is the first non-bovine isolate obtained from the mosquito vector . We further compared these MPOV sequences with known sequences of the Yunnan orbivirus and other known orbivirus sequences of mosquito origin found in Australia. The phylogenetic analyses indicate the Australian MPOV sequences are more closely related to each other than other known sequences of Yunnan orbivirus. Furthermore, MPOV sequences are closely related to sequences from the Indonesian isolate JKT-8650. The clustering of Australian sequences in the phylogenetic tree suggests the monophyletic lineage of MPOV circulating in Australia. Further, ongoing surveillance is required to assess the existence and prevalence of this or other yet undetected lineages of MPOV and other orbiviruses in Australia.
中间点病毒（MPOV）是一种澳大利亚虫媒病毒，属于在中国发现的 种。MPOV 首次在北领地（NT）的比阿特丽斯山被发现和报告，迄今为止，仅在澳大利亚的 NT 被独家报告。虽然以前已经对 MPOV 的遗传特征进行了描述，但仅限于分别编码核心蛋白 VP2 和外壳蛋白 VP3 的片段 2 和 3 的序列信息。本研究首次展示了 MPOV 的几乎全长基因组序列，这些序列代表了 1997 年至 2018 年 20 多年间从 24 个分离株收集的序列。虽然大多数分离株是在 MPOV 最常分离的北领地比阿特丽斯山采样的，但本报告还描述了 MPOV 从澳大利亚昆士兰州（QLD）的首次两次分离。其中之一是从蚊子媒介中获得的第一个非牛分离株 。我们进一步将这些 MPOV 序列与已知的云南虫媒病毒序列和在澳大利亚发现的其他已知蚊源虫媒病毒序列进行了比较。系统发育分析表明，澳大利亚的 MPOV 序列彼此之间的关系比其他已知的云南虫媒病毒序列更为密切。此外，MPOV 序列与来自印度尼西亚分离株 JKT-8650 的序列密切相关。系统发育树中澳大利亚序列的聚类表明，MPOV 在澳大利亚循环的单系谱系。此外，需要进行持续监测，以评估这种或其他尚未检测到的 MPOV 和其他虫媒病毒谱系在澳大利亚的存在和流行情况。
