Vector-borne Viral Diseases Programme, Institute for Animal Health, Woking, Surrey, United Kingdom.
PLoS One. 2012;7(3):e31911. doi: 10.1371/journal.pone.0031911. Epub 2012 Mar 15.
Eubenangee virus has previously been identified as the cause of Tammar sudden death syndrome (TSDS). Eubenangee virus (EUBV), Tilligery virus (TILV), Pata virus (PATAV) and Ngoupe virus (NGOV) are currently all classified within the Eubenangee virus species of the genus Orbivirus, family Reoviridae. Full genome sequencing confirmed that EUBV and TILV (both of which are from Australia) show high levels of aa sequence identity (>92%) in the conserved polymerase VP1(Pol), sub-core VP3(T2) and outer core VP7(T13) proteins, and are therefore appropriately classified within the same virus species. However, they show much lower amino acid (aa) identity levels in their larger outer-capsid protein VP2 (<53%), consistent with membership of two different serotypes - EUBV-1 and EUBV-2 (respectively). In contrast PATAV showed significantly lower levels of aa sequence identity with either EUBV or TILV (with <71% in VP1(Pol) and VP3(T2), and <57% aa identity in VP7(T13)) consistent with membership of a distinct virus species. A proposal has therefore been sent to the Reoviridae Study Group of ICTV to recognise 'Pata virus' as a new Orbivirus species, with the PATAV isolate as serotype 1 (PATAV-1). Amongst the other orbiviruses, PATAV shows closest relationships to Epizootic Haemorrhagic Disease virus (EHDV), with 80.7%, 72.4% and 66.9% aa identity in VP3(T2), VP1(Pol), and VP7(T13) respectively. Although Ngoupe virus was not available for these studies, like PATAV it was isolated in Central Africa, and therefore seems likely to also belong to the new species, possibly as a distinct 'type'. The data presented will facilitate diagnostic assay design and the identification of additional isolates of these viruses.
伊蚊病毒先前被认为是塔玛氏猝死综合征（TSDS）的致病因子。伊蚊病毒（EUBV）、蒂利杰里病毒（TILV）、帕塔病毒（PATAV）和恩古佩病毒（NGOV）目前均归类于呼肠孤病毒科正呼肠孤病毒属的伊蚊病毒种。全基因组测序证实，EUBV 和 TILV（均来自澳大利亚）在保守聚合酶 VP1（Pol）、亚核心 VP3（T2）和外壳蛋白 VP7（T13）蛋白中具有高 aa 序列同一性（>92%），因此适当归类于同一病毒种。然而，它们在较大的外壳蛋白 VP2 中具有低得多的氨基酸（aa）同一性（<53%），与两种不同血清型一致-EUBV-1 和 EUBV-2（分别）。相比之下，PATAV 与 EUBV 或 TILV 的 aa 序列同一性明显较低（VP1（Pol）和 VP3（T2）<71%，VP7（T13）<57%aa 同一性），与独特病毒种一致。因此，已向 ICTV 呼肠孤病毒研究小组提交了一项提案，将“帕塔病毒”确认为一种新的呼肠孤病毒种，PATAV 分离株为血清型 1（PATAV-1）。在其他呼肠孤病毒中，PATAV 与马传贫病毒（EHDV）的亲缘关系最密切，VP3（T2）、VP1（Pol）和 VP7（T13）的 aa 同一性分别为 80.7%、72.4%和 66.9%。尽管无法获得恩古佩病毒进行这些研究，但与 PATAV 一样，它也是在中非分离的，因此似乎也属于新种，可能是一种独特的“类型”。所提交的数据将有助于诊断检测设计和鉴定这些病毒的其他分离株。
