人类原始多能干细胞的维持。

Maintenance of Human Naïve Pluripotent Stem Cells.

机构信息

Epigenetics Programme, Babraham Institute, Cambridge, UK.

出版信息

Methods Mol Biol. 2022;2416:73-90. doi: 10.1007/978-1-0716-1908-7_6.

DOI:10.1007/978-1-0716-1908-7_6

Abstract

Naïve pluripotent stem cells are the in vitro counterparts of pre-implantation embryonic epiblast. During the last few years, several protocols for establishing and maintaining human pluripotent stem cells (hPSCs) with naïve features have been reported, and many of these protocols result in cell populations with different molecular characteristics. As such, choosing the most appropriate method for naïve hPSC maintenance can pose a significant challenge. This chapter presents an optimized system called PXGL for culturing naïve hPSCs. Naïve hPSCs robustly self-renew while retaining a normal karyotype in PXGL, and the protocol is reproducible across different cell lines and independent laboratories.

摘要

原始多能干细胞是体外类胚层的对应物。在过去的几年中，已经报道了几种建立和维持具有原始特征的人类多能干细胞（hPSC）的方案，其中许多方案导致具有不同分子特征的细胞群体。因此，选择最合适的原始 hPSC 维持方法可能是一项重大挑战。本章介绍了一种称为 PXGL 的优化系统，用于培养原始 hPSC。原始 hPSC 在 PXGL 中强大地自我更新，同时保持正常核型，该方案在不同的细胞系和独立的实验室中具有可重复性。