State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiangan South Road, Xiamen 361102, Fujian, China.
The Plastic And Aesthetic Burn Department, The First affiliated Hospital of Xiamen University, Xiamen 361003, Fujian Province, China; Xiamen Humanity Hospital, Fujian Medical University, Xianyue Road, Xiamen 361009, Fujian, China.
Life Sci. 2022 Jan 1;288:120205. doi: 10.1016/j.lfs.2021.120205. Epub 2021 Dec 3.
This study was aimed to explore whether sacran polysaccharide has a therapeutic effect on atopic dermatitis (AD) and its possible mechanisms.
2, 4-dinitrochlorobenzene (DNCB)-induced AD mice were treated with 0.2% Sacran, 0.5% Sacran and 0.1% tacrolimus. Through scoring dermatitis severity, measuring ear thickness, cracking behavior, open field test, we evaluated the therapeutic effect of Sacran on DNCB-induced AD mice. CD4 T cells and CD8 T cells were evaluated by flow cytometry. The relative expression of Ifng and Il4 were measured by real-time quantitative PCR.
Sacran could relieved the symptoms of DNCB-induced AD mice, such as AD score, ear thickness, and IgE release. Sacran may alleviate dermatitis by inhibiting Th2 activation and reducing IgE release.
Our research further proved that polysaccharide Sacran has anti-dermatitis effects, and also clarified its mechanism of alleviating dermatitis by inhibiting the activation of Th2 cells and reducing the release of IgE, which provides a theoretical basis for the future clinical transformation of polysaccharide Sacran.
本研究旨在探讨壳聚糖多糖是否对特应性皮炎（AD）具有治疗作用及其可能的机制。
用 2,4-二硝基氯苯（DNCB）诱导 AD 小鼠，用 0.2%壳聚糖、0.5%壳聚糖和 0.1%他克莫司进行治疗。通过评分皮炎严重程度、测量耳厚度、开裂行为、旷场试验，评估壳聚糖对 DNCB 诱导的 AD 小鼠的治疗效果。通过流式细胞术评估 CD4 T 细胞和 CD8 T 细胞。通过实时定量 PCR 测量 Ifng 和 Il4 的相对表达。
壳聚糖可缓解 DNCB 诱导的 AD 小鼠的症状，如 AD 评分、耳厚和 IgE 释放。壳聚糖可能通过抑制 Th2 激活和减少 IgE 释放来缓解皮炎。
我们的研究进一步证明了多糖壳聚糖具有抗皮炎作用，并阐明了其通过抑制 Th2 细胞的激活和减少 IgE 的释放来缓解皮炎的机制，为多糖壳聚糖未来的临床转化提供了理论依据。
