猪粪促进了抗生素耐药组，包括对替加环素耐药的 tet(X)变体，向农场工人和接收环境的传播。
Swine manure facilitates the spread of antibiotic resistome including tigecycline-resistant tet(X) variants to farm workers and receiving environment.
机构信息
Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China; National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, South China Agricultural University, Guangzhou 510642, China; National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China; National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, South China Agricultural University, Guangzhou 510642, China; National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China.
出版信息
Sci Total Environ. 2022 Feb 20;808:152157. doi: 10.1016/j.scitotenv.2021.152157. Epub 2021 Dec 4.
The prevalence of antibiotic resistance genes (ARGs) in livestock and poultry manure is a severe threat to human health. However, the comprehensive characterization of antibiotic resistance in swine, workers, and the receiving environment is still lacking in the actual breeding environment. Hence, the ARG profile and the potential bacterial hosts producing among swine manure (including sows, piglets, finishing pigs, and nursery pigs), worker feces, and the receiving environment (including sediment and vegetable soil) were comprehensively analyzed based on the metagenomic method. The results showed that swine manure exhibited the high levels of richness and diversity of ARGs. Inactivating tetracycline resistance genes such as tet(X), tet(X1), and tet(X10) were prevalent on swine farms. Workers and the environment were the primary recipients of ARGs, and shared ARGs accounted for at least 90% of their ARG abundances. Network analysis revealed that Escherichia, Acinetobacter, and Erysipelothrix were the most dominant genera co-occurring with specific shared ARGs. The abundance of coexisting ARGs in swine at different developmental stages accounted for 76.4% to 90.8% of the shared ARGs in swine, workers, and environmental samples. The Mantel test revealed that Firmicutes and Proteobacteria had a significant correlation with the ARG profiles. In addition, variation partitioning analysis (VPA) showed that the joint effects of mobile genetic elements (MGEs) and bacterial communities accounted for 24.7% of the resistome variation and played a significant role in the ARG profiles. These results improve our understanding of the transmission and persistence of ARGs in the actual breeding environment.
抗生素耐药基因（ARGs）在禽畜粪便中的存在是对人类健康的严重威胁。然而，在实际养殖环境中，对猪、工人和受纳环境中抗生素耐药性的综合特征描述仍然缺乏。因此，本研究采用宏基因组学方法，全面分析了猪粪（包括母猪、仔猪、育肥猪和保育猪）、工人粪便和受纳环境（包括沉积物和菜园土）中的 ARG 谱和可能产生的细菌宿主。结果表明，猪粪表现出丰富多样的 ARG 水平。在养猪场中，四环素类耐药基因如 tet(X)、tet(X1)和 tet(X10)处于优势地位。工人和环境是 ARG 的主要接受者，共享 ARG 至少占其 ARG 丰度的 90%。网络分析表明，Escherichia、Acinetobacter 和 Erysipelothrix 是与特定共享 ARG 最相关的主要共存属。不同发育阶段猪共存 ARG 的丰度占猪、工人和环境样品中共享 ARG 的 76.4%至 90.8%。Mantel 检验表明，Firmicutes 和 Proteobacteria 与 ARG 谱具有显著相关性。此外，变异分解分析（VPA）表明，移动遗传元件（MGEs）和细菌群落的联合效应解释了 24.7%的耐药组变异，对 ARG 谱具有重要作用。这些结果提高了我们对实际养殖环境中 ARGs 传播和持久性的认识。
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