Improved performance and microbial community dynamics in anaerobic fermentation of triticale silages at different stages.

Production of high-quality grass-based silages by microbial-mediated anaerobic fermentation is an effective strategy in livestock farms. In the present study, an ensiling process was used to preserve and enhance fermentative metabolites in triticale silages with novel inoculants of Lactobacillus rhamanosus -52 and, Lactobacillus rhamanosus-54. Triticale silages treated with LAB predominantly had lower pH values than control silages due to rapid changes of microbial counts. LAB addition improved anaerobic fermentation profiles showing higher lactic acid, but lower acetic acid and butyric acid concentrations. A background microbial dynamic study indicated that the addition of L. rhamanosus-52 and L. rhamanosus-54 improved silage fermentation, enriched Lactobacillus spp., and decreased microbial richness with diversity, leading to increased efficiency of lactic acid fermentation. In conclusion, LAB treatment can increase silage quality by enhancing the dominance of desirable Lactobacillus while inhibiting the growth of undesirable microbes.