新型含纳米磷酸钙的纳米复合树脂渗透剂预防釉质白斑

Novel nanostructured resin infiltrant containing calcium phosphate nanoparticles to prevent enamel white spot lesions.

机构信息

Department of Orthodontics, School of Stomatology, Capital Medical University, Beijing, China.

Department of Endodontics, Periodontics and Prosthodontics, University of Maryland Dental School, Baltimore, MD, 21201, USA.

出版信息

J Mech Behav Biomed Mater. 2022 Feb;126:104990. doi: 10.1016/j.jmbbm.2021.104990. Epub 2021 Nov 23.

DOI:10.1016/j.jmbbm.2021.104990

PMID:34871957

Abstract

OBJECTIVES

The objective of this study was to develop a novel nanostructured resin infiltrant containing nanoparticles of amorphous calcium phosphate (NACP) to treat enamel white spot lesions (WSLs). Physical properties and the therapeutic effect of the new resin infiltrant were investigated for the first time.

METHODS

NACP was incorporated into ICON (Icon caries infiltrant, DMG, Germany) with different mass fractions. Cytotoxicity, degree of conversion, surface hardness, calcium (Ca) and phosphorus (P) ions release concentrations were tested. After application to the demineralized enamel samples, the color changes were determined. Surface and cross-sectional hardness were measured, scanning electron microscopy (SEM) images were taken on the cross-section of samples to observe microstructure changes after 14-day pH cycling.

RESULTS

Incorporating 10%-30% of NACP did not compromise the biocompatibility and physical properties of the resin infiltrant. ICON + 30% NACP group had long-lasting and high level of Ca and P ion release. After 14-day pH cycling, enamel surface hardness of ICON + 30% NACP group was 1.83 ± 0.21 GPa, significantly higher than the control group (1.32 ± 0.18 GPa) (p < 0.05). ICON + 30NACP group had the highest cross-sectional enamel hardness among all groups (p < 0.05), especially at 50 μm and 100 μm depth. SEM images showed that apparent enamel prism and inter-prism gaps in negative control were masked by mineral deposition in ICON + 30% NACP group.

SIGNIFICANCE

The novel ICON+30% NACP infiltrant is promising to inhibit enamel WSLs, protect the enamel and increase its hardness.

摘要

目的

本研究旨在开发一种新型的纳米结构树脂渗透剂，其中包含纳米级无定形磷酸钙（NACP）颗粒，以治疗釉质白斑（WSL）。首次研究了这种新型树脂渗透剂的物理性能和治疗效果。

方法

将 NACP 以不同的质量分数掺入 ICON（Icon 龋病渗透剂，DMG，德国）中。测试细胞毒性、转化率、表面硬度、钙（Ca）和磷（P）离子释放浓度。将处理过的脱矿釉质样本进行应用后，测定其颜色变化。测量表面和横截面硬度，对样本的横截面进行扫描电子显微镜（SEM）图像拍摄，观察 pH 循环 14 天后的微观结构变化。

结果

掺入 10%-30%的 NACP 不会影响树脂渗透剂的生物相容性和物理性能。ICON + 30% NACP 组具有持久且高水平的 Ca 和 P 离子释放。经过 14 天的 pH 循环后，ICON + 30% NACP 组的釉质表面硬度为 1.83 ± 0.21 GPa，明显高于对照组（1.32 ± 0.18 GPa）（p < 0.05）。在所有组中，ICON + 30NACP 组的横截面釉质硬度最高（p < 0.05），特别是在 50 μm 和 100 μm 深度处。SEM 图像显示，在 ICON + 30% NACP 组中，明显的釉质棱柱体和棱柱间间隙被矿化沉积所掩盖。