Buus S, Werdelin O
Acta Pathol Microbiol Immunol Scand C. 1986 Feb;94(1):17-24. doi: 10.1111/j.1699-0463.1986.tb02084.x.
We have studied if antigens of different size and structure all require processing in antigen-presenting cells of guinea-pigs before they can be recognized by T cells. The method of mild paraformaldehyde fixation was used to stop antigen-processing in the antigen-presenting cells. As a measure of antigen presentation we used the proliferative response of appropriately primed T cells during a co-culture with the paraformaldehyde-fixed and antigen-exposed presenting cells. We demonstrate that the large synthetic polypeptide antigen, dinitrophenyl-poly-L-lysine, requires processing. After an initial time-lag of 30 min this antigen is fully processed within 2 to 4 of culture at 37 degrees C. In contrast, the immunogenic heptapeptide, angiotensin III, can be presented by pre-fixed accessory cells, viz. without any prior processing. Antigen processing was found to be temperature-dependent and consequently energy-requiring. Processing is strongly inhibited by the lysosomotrophic drug, chloroquine, suggesting a lysosomal involvement in antigen processing. The existence of a minor, non-lysosomal pathway is suggested, since small amounts of antigen were processed even at 10 degrees C, at which temperature no transport to and from the lysosomes can occur.
我们研究了不同大小和结构的抗原在被豚鼠的抗原呈递细胞处理之前，是否都需要经过处理才能被T细胞识别。采用轻度多聚甲醛固定的方法来终止抗原呈递细胞中的抗原处理过程。作为抗原呈递的衡量指标，我们使用了经过适当致敏的T细胞在与经多聚甲醛固定并暴露于抗原的呈递细胞共培养期间的增殖反应。我们证明，大的合成多肽抗原二硝基苯基 - 聚 - L - 赖氨酸需要经过处理。在最初30分钟的时间延迟后，该抗原在37℃培养2至4小时内被完全处理。相比之下，免疫原性七肽血管紧张素III可以由预先固定的辅助细胞呈递，即无需任何预先处理。发现抗原处理是温度依赖性的，因此需要能量。溶酶体营养药物氯喹强烈抑制处理过程，这表明溶酶体参与了抗原处理。由于即使在10℃时仍有少量抗原被处理，而在此温度下不会发生与溶酶体的运输往来，因此提示存在一条次要的、非溶酶体途径。
