特定抗核抗体的补体激活能力。
Complement-activating abilities of defined antinuclear antibodies.
作者信息
Kanayama Y, Peebles C, Tan E M, Curd J G
出版信息
Arthritis Rheum. 1986 Jun;29(6):748-54. doi: 10.1002/art.1780290607.
A complement-fixing immunofluorescence assay on HEp-2 cells was used to assess the ability of various antinuclear antibodies (ANA) to activate complement. Sera which contained only specific antibodies to nuclear RNP, SS-B/La, centromere, Sm antigen, double-stranded DNA, and/or nuclear histone were selected. Relative abilities of various ANA to activate complement were determined from the ratio of titers of C3, C4, or properdin-fixing ANA to the IgG ANA titers. Nuclear RNP-anti-RNP complexes activated and deposited significantly more complement C3 than other ANA (P less than 0.02). Antibodies to SS-B/La, centromere, and Sm activated more complement than anti-DNA or antihistone (P less than 0.02). Antihistone antibodies activated the least complement. These studies demonstrate that different ANA have significantly different orders of complement-activating capabilities when bound to their respective nuclear antigens.
采用针对人喉癌上皮细胞（HEp-2细胞）的补体结合免疫荧光试验，评估各种抗核抗体（ANA）激活补体的能力。选取仅含有针对核核糖核蛋白（RNP）、SS-B/La、着丝粒、Sm抗原、双链DNA和/或核组蛋白特异性抗体的血清。通过C3、C4或备解素结合ANA的滴度与IgG ANA滴度之比，确定各种ANA激活补体的相对能力。与其他ANA相比，核RNP-抗RNP复合物激活并沉积的补体C3明显更多（P<0.02）。与抗DNA或抗组蛋白抗体相比，针对SS-B/La、着丝粒和Sm的抗体激活的补体更多（P<0.02）。抗组蛋白抗体激活的补体最少。这些研究表明，不同的ANA与各自的核抗原结合时，其补体激活能力的顺序存在显著差异。
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