Department of Orthopedic Surgery, Hamamatsu University School of Medicine, 1-20-1, Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan.
Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1, Handayama, Higashi-ku, Hamamatsu-city, Shizuoka, 431-3192, Japan.
Sci Rep. 2021 Dec 6;11(1):23515. doi: 10.1038/s41598-021-02818-7.
Ligamentum flavum hypertrophy (HLF) is the most important component of lumbar spinal canal stenosis (LSCS). Analysis of hypertrophied ligamentum flavum (HLF) samples from patients with LSCS can be an important que. The current study analyzed the surgical samples of HLF samples in patients with LCSC using quantitative and qualitative high performance-liquid chromatography and mass spectrometry. We collected ligamentum flavum (LF) tissue from twelve patients with LSCS and from four patients with lumbar disk herniation (LDH). We defined LF from LSCS patients as HLF and that from LDH patients as non-hypertrophied ligamentum flavum (NHLF). Total lipids were extracted from the LF samples and evaluated for quantity and quality using liquid chromatography and mass spectrometry. The total lipid amount of the HLF group was 3.6 times higher than that of the NHLF group. Phosphatidylcholines (PCs), ceramides (Cers), O-acyl-ω-hydroxy fatty acids (OAHFAs), and triglycerides (TGs) in the HLF group were more than 32 times higher than those of the NHLF group. PC(26:0)+H+, PC(25:0)+H+, and PC(23:0)+H+ increased in all patients in the HLF group compared to the NHLF group. The thickness of the LF correlated significantly with PC(26:0)+H+ in HLF. We identified the enriched specific PCs, Cers, OAHFAs, and TGs in HLF.
黄韧带肥厚（HLF）是腰椎管狭窄症（LSCS）最重要的组成部分。分析 LSCS 患者肥厚黄韧带（HLF）样本可以作为一个重要的指标。本研究采用定量和定性高效液相色谱-质谱联用技术对 LSCS 患者的 HLF 样本进行了分析。我们收集了 12 例 LSCS 患者和 4 例腰椎间盘突出症（LDH）患者的黄韧带（LF）组织。我们将 LSCS 患者的 LF 定义为 HLF，将 LDH 患者的 LF 定义为非肥厚黄韧带（NHLF）。从 LF 样本中提取总脂质，并通过液相色谱和质谱法评估其数量和质量。HLF 组的总脂质量是 NHLF 组的 3.6 倍。HLF 组的磷脂酰胆碱（PCs）、神经酰胺（Cers）、O-酰基-ω-羟基脂肪酸（OAHFAs）和甘油三酯（TGs）含量均高于 NHLF 组的 32 倍以上。与 NHLF 组相比，所有 HLF 组患者的 PC（26:0）+H+、PC（25:0）+H+和 PC（23:0）+H+均增加。HLF 组 LF 的厚度与 PC（26:0）+H+呈显著正相关。我们在 HLF 中鉴定出了富含特定 PC、Cers、OAHFAs 和 TGs。
