Cecchelli R, Cacan R, Porchet-Hennere E, Verbert A
Biosci Rep. 1986 Feb;6(2):227-34. doi: 10.1007/BF01115011.
Incubation of mouse thymocytes with 10 microM monensin for 1 hour induces morphological alterations characterized by the extensive dilatation and vacuolization of the Golgi complex. This effect is used to study the transport and utilization of labelled sugar nucleotides into intracellular vesicles by using thymocytes whose plasma membrane has been permeabilized by ammonium chloride treatment. It is demonstrated that monensin stimulates the incorporation of labelled sialyl, fucosyl, galactosyl, and N-acetylglucosaminyl residues. This enhanced incorporation is not due to a direct effect of monensin on glycosyltransferase activities themselves but is a consequence of a higher entry and accumulation of labelled sugar nucleotides in the dilated vesicles.
将小鼠胸腺细胞与10微摩尔莫能菌素孵育1小时会诱导形态学改变，其特征为高尔基体复合体广泛扩张和空泡化。利用氯化铵处理使质膜通透性增加的胸腺细胞，此效应被用于研究标记糖核苷酸向细胞内囊泡的转运和利用。结果表明，莫能菌素刺激标记的唾液酸基、岩藻糖基、半乳糖基和N - 乙酰葡糖胺基残基的掺入。这种掺入增强并非莫能菌素对糖基转移酶活性本身的直接作用，而是标记糖核苷酸在扩张囊泡中更高的进入和积累的结果。
