Ellinger A, Pavelka M
Cell Tissue Res. 1984;235(1):187-94. doi: 10.1007/BF00213739.
The effect of short-time treatment with the ionophore monensin, administered intraluminally at concentrations of 5 and 10 microM, was studied on the Golgi apparatus of absorptive cells in the small intestine of the rat. At 2-3 min after treatment most of the Golgi stacks exhibited dilated cisternae. At 4-5 min stacked cisternae were absent; they were replaced by groups of smooth-surfaced vacuoles. Dilatation and vacuolization occurred in the entire stacks without preferential effect on any particular Golgi subcompartment. Monensin did not influence the cytochemical Golgi reaction of thiamine pyrophosphatase and acid phosphatase. The characteristic staining pattern of these two enzymes in all Golgi cisternae of absorptive cells in the proximal small intestine, and the reactivity restricted to trans cisternae in distal segments of the small intestine, were unchanged after treatment with monensin. In the distal small intestine, the cytochemical pattern allowed the monensin-induced vacuoles to be attributed to the former cis- or trans-Golgi face. Further, the cytochemical results demonstrate that vacuolization is not restricted to the stacked cisternae, but includes the trans-most cisterna. The latter, usually located at some distance from the Golgi stacks, has been defined as belonging to the GERL system in several types of cells. The clear response to monensin, an agent that selectively affects the Golgi apparatus, indicates common properties between trans-most and stacked Golgi cisternae.
研究了以5和10微摩尔浓度腔内给予离子载体莫能菌素进行短时间处理对大鼠小肠吸收细胞高尔基体的影响。处理后2 - 3分钟，大多数高尔基体堆叠显示出扩张的扁平囊。4 - 5分钟时，堆叠的扁平囊消失；它们被一组组表面光滑的液泡所取代。扩张和空泡化发生在整个堆叠中，对任何特定的高尔基体亚区没有优先影响。莫能菌素不影响硫胺焦磷酸酶和酸性磷酸酶的细胞化学高尔基体反应。在用莫能菌素处理后，近端小肠吸收细胞所有高尔基体扁平囊中这两种酶的特征染色模式以及在小肠远端段仅限于反式扁平囊的反应性均未改变。在小肠远端，细胞化学模式使莫能菌素诱导的液泡可归因于以前的顺式或反式高尔基体面。此外，细胞化学结果表明空泡化不仅限于堆叠的扁平囊，还包括最远端的扁平囊。后者通常位于距高尔基体堆叠有一定距离处，在几种类型的细胞中已被定义为属于GERL系统。对选择性影响高尔基体的试剂莫能菌素的明显反应表明最远端和堆叠的高尔基体扁平囊之间具有共同特性。
