Brown R D, Yuen E, Kronenberg H, Rickard K A
Blood. 1986 Jul;68(1):37-40.
Cord plasma contains colony-stimulating activity (CSA) which stimulates the in vitro clonal growth of neutrophils, eosinophils, macrophages, erythrocytes, and persisting mast cells in semisolid cultures. Analysis of day 35 colonies in agar cultures was found to be a suitable means of demonstrating this activity and discriminating between it and granulocyte-macrophage colony-stimulating factor (GM-CSF). Serum (10%) from patients with acute and chronic myeloid leukemia (AML and CML) was added to normal human bone marrow cultures to search for similar activity in these patient's serum. Although the number of colonies on day 12 (predominantly neutrophils and macrophages) was not significantly different from the number of colonies in cultures containing normal serum, the number of colonies increased 500% in cultures containing CML serum on day 35. Serum from patients with AML during regeneration also stimulated an increased number of colonies on day 35. Although both eosinophil and mast cell colonies were still present on day 35, only mast cell colonies persisted for 150 days. On day 35, cultures containing 10% CML serum contained predominantly eosinophil colonies (84%), whereas cultures containing AML serum contained predominantly mast cell colonies (76%). Although serum contains various CSFs, the specific factor which stimulates persisting mast cell colonies may be the human equivalent of murine persisting (P) cell-stimulating factor (Multi-CSF).
脐血血浆含有集落刺激活性（CSA），它能刺激中性粒细胞、嗜酸性粒细胞、巨噬细胞、红细胞和持续存在的肥大细胞在半固体培养物中的体外克隆生长。琼脂培养物中第35天集落的分析被发现是证明这种活性并将其与粒细胞-巨噬细胞集落刺激因子（GM-CSF）区分开来的合适方法。将急性和慢性髓系白血病（AML和CML）患者的血清（10%）添加到正常人骨髓培养物中，以寻找这些患者血清中的类似活性。尽管第12天的集落数量（主要是中性粒细胞和巨噬细胞）与含有正常血清的培养物中的集落数量没有显著差异，但在含有CML血清的培养物中，第35天的集落数量增加了500%。再生期AML患者的血清在第35天也刺激了集落数量的增加。尽管在第35天嗜酸性粒细胞和肥大细胞集落仍然存在，但只有肥大细胞集落持续了150天。在第35天，含有10% CML血清的培养物主要含有嗜酸性粒细胞集落（84%），而含有AML血清的培养物主要含有肥大细胞集落（76%）。尽管血清含有多种集落刺激因子，但刺激持续存在的肥大细胞集落的特定因子可能是与小鼠持续存在（P）细胞刺激因子（多集落刺激因子）等效的人类因子。
