Brown R D, Yuen E, Rickard K A, Kronenberg H
Leuk Res. 1987;11(6):545-7. doi: 10.1016/0145-2126(87)90090-7.
Addition of serum from patients with chronic myeloid leukemia (CML) in both chronic phase and blastic transformation, to agar cultures of normal human marrow cells stimulated the growth of persisting colonies (day 35) containing either eosinophils or mast cells. Chronic phase serum stimulated an 800% increase in the total number of these colonies of which only 16% were mast cells. Serial studies using serum from 2 patients demonstrated that the proportion of mast cells increased during the progression to blastic transformation. The emergence of a greater proportion of persisting mast cell colonies and a decrease in absolute number of eosinophil colonies in agar cultures of normal marrow cells containing serum from patients with CML coincides with the emergence of blastic transformation and suggests that a significant change occurs in the absolute and relative concentration of hemopoietic growth factors in these patients.
将慢性期和急变期慢性髓性白血病（CML）患者的血清添加到正常人骨髓细胞的琼脂培养物中，可刺激含有嗜酸性粒细胞或肥大细胞的持续菌落（第35天）生长。慢性期血清刺激这些菌落总数增加800%，其中只有16%是肥大细胞。对2例患者血清进行的系列研究表明，在进展为急变期的过程中，肥大细胞的比例增加。在含有CML患者血清的正常骨髓细胞琼脂培养物中，持续存在的肥大细胞菌落比例增加，嗜酸性粒细胞菌落绝对数量减少，这与急变期的出现相吻合，表明这些患者造血生长因子的绝对和相对浓度发生了显著变化。
