Drexler H G, Gaedicke G, Novotny J, Minowada J
Cancer. 1986 Jul 15;58(2):245-51. doi: 10.1002/1097-0142(19860715)58:2<245::aid-cncr2820580208>3.0.co;2-4.
The isoenzyme profiles of hexosaminidase (N-acetyl-beta-D-glucosaminidase) were analyzed by isoelectric focusing on horizontal polyacrylamide thin-layer gel with special emphasis on the intermediate isoenzyme (Hex I). The expression of Hex I was examined in 87 leukemia-lymphoma cell lines, in 14 B-lymphoblastoid cell lines, in 441 cases of leukemia-lymphoma (specimens containing 80% or more tumor cells), in 22 leukemia cell lines and in 14 cases of leukemia that had been treated with phorbolesters (TPA) for induction of differentiation, and in the mononuclear cell preparations separated from peripheral blood, lymph node, thymus, bone marrow, tonsil, liver, and spleen specimens from normal donors. Hex I was detected in the leukemia cell lines arrested at early, immature or at late, mature stages of B- and T-cell differentiation, but not in cell lines blocked at intermediate stages of maturation. Most myelomonocytic leukemia cell lines and the erythroleukemia cell lines showed Hex I, whereas the B-lymphoblastoid cell lines were negative for this marker. During induction of differentiation, the expression of Hex I was lost in 13 of 15 leukemia cell lines that were originally Hex I-positive. Among the panel of the "fresh" leukemia-lymphoma cells, Hex I was found predominantly in cases of acute lymphoblastic leukemia and acute myeloblastic/monoblastic leukemia, but rarely or not at all in the mature T-, B- or myeloid malignancies. However, two out of two cases of multiple myeloma were Hex I-positive, and the Hex I expression could be induced by TPA in three of six B-cell chronic lymphocytic leukemia cases. Chronic myelocytic leukemia cells remained Hex I-negative during induction of differentiation. Hex I-positivity was not detected in the cell preparations from normal tissues, and peripheral blood indicating that the normal cellular counterpart of the Hex I-positive tumor cells are present at only low percentages within the respective cell populations. It is suggested that Hex I is a marker of early lymphoid and myeloid hematopoiesis that is no longer expressed in intermediate stages of lymphoid differentiation and in later or terminal stages of myeloid differentiation, but that is again detectable in terminally differentiated B-cells. Further studies will focus on identification and isolation of normal Hex I-positive cells.
通过在水平聚丙烯酰胺薄层凝胶上进行等电聚焦分析己糖胺酶（N - 乙酰 - β - D - 氨基葡萄糖苷酶）的同工酶谱，特别关注中间同工酶（Hex I）。在87株白血病 - 淋巴瘤细胞系、14株B淋巴母细胞系、441例白血病 - 淋巴瘤（肿瘤细胞含量80%或更多的标本）、22株白血病细胞系以及14例经佛波酯（TPA）诱导分化治疗的白血病病例中检测Hex I的表达，并在从正常供体的外周血、淋巴结、胸腺、骨髓、扁桃体、肝脏和脾脏标本中分离得到的单核细胞制剂中检测。在停滞于B细胞和T细胞分化早期、未成熟或晚期、成熟阶段的白血病细胞系中检测到Hex I，但在成熟中间阶段受阻的细胞系中未检测到。大多数髓单核细胞白血病细胞系和红白血病细胞系显示Hex I阳性，而B淋巴母细胞系对此标记物呈阴性。在诱导分化过程中，最初Hex I阳性的15株白血病细胞系中有13株Hex I表达丧失。在“新鲜”白血病 - 淋巴瘤细胞组中，Hex I主要见于急性淋巴细胞白血病和急性髓细胞性/单核细胞性白血病病例，但在成熟的T细胞、B细胞或髓系恶性肿瘤中很少或根本未发现。然而，两例多发性骨髓瘤病例中有两例Hex I阳性，并且在六例B细胞慢性淋巴细胞白血病病例中有三例Hex I表达可被TPA诱导。慢性粒细胞白血病细胞在诱导分化过程中仍为Hex I阴性。在正常组织和外周血的细胞制剂中未检测到Hex I阳性，这表明Hex I阳性肿瘤细胞的正常细胞对应物在各自细胞群体中仅占低百分比。提示Hex I是早期淋巴样和髓样造血的标志物，在淋巴样分化的中间阶段以及髓样分化的后期或终末阶段不再表达，但在终末分化的B细胞中再次可检测到。进一步的研究将集中于正常Hex I阳性细胞的鉴定和分离。
