Castilhos Janaína Kehl de, Campagnolo Paula Dal Bó, Almeida Silvana de, Vitolo Márcia Regina, Mattevi Vanessa Suñé
Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil.
Universidade do Vale do Rio dos Sinos, São Leopoldo, RS, Brazil.
Genet Mol Biol. 2021 Dec 3;44(4):e20200330. doi: 10.1590/1678-4685-GMB-2020-0330. eCollection 2021.
Epigenetic modifications established during prenatal and early life, including DNA methylation, have been suggested as potential mediators of the interaction between environmental exposures during the perinatal period and adult metabolic health adverse outcomes, especially cardiometabolic complications and overweight. The effect of a dietary intervention in the first year of life on global methylation levels in leukocyte samples from a cohort of children born between 2001 and 2002 in southern Brazil was examined. Overall methylation measurements were performed using enzyme-linked immunosorbent assays on DNA samples from 237 children at 4 years old. Mean methylation values were higher in the intervention group (mean: 2.20 ± 1.31%) than in the control group (mean: 1.65 ± 1.11%; P = 0.001). It was observed that nutritional counseling in the first year increased breastfeeding duration and stimulated the development of healthier eating habits. Therefore, these factors might have contributed to increase global DNA methylation. The findings of the present study reinforce the notion that performing nutritional interventions in the early stages of life is important and provide further evidence of the interaction between the environment and epigenetic traits.
产前和生命早期建立的表观遗传修饰，包括DNA甲基化，被认为是围产期环境暴露与成人代谢健康不良后果（尤其是心脏代谢并发症和超重）之间相互作用的潜在介质。研究了巴西南部2001年至2002年出生的一组儿童生命第一年的饮食干预对白细胞样本中整体甲基化水平的影响。使用酶联免疫吸附测定法对237名4岁儿童的DNA样本进行了整体甲基化测量。干预组的平均甲基化值（平均值：2.20±1.31%）高于对照组（平均值：1.65±1.11%；P = 0.001）。研究发现，生命第一年的营养咨询增加了母乳喂养时间，并促进了更健康饮食习惯的养成。因此，这些因素可能有助于增加整体DNA甲基化。本研究结果强化了在生命早期进行营养干预很重要的观念，并为环境与表观遗传特征之间的相互作用提供了进一步的证据。
