Clinic for Veterinary Obstetrics, Gynaecology and Andrology for Large and Small Animals with Veterinary Ambulance, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Giessen, Germany.
Unit for Biomathematics and Data Processing, Justus-Liebig-University Giessen, Giessen, Germany.
Vet Med Sci. 2022 Jan;8(1):157-163. doi: 10.1002/vms3.681. Epub 2021 Dec 7.
Epididymal sperm can be collected post-castration or post-mortem. This method has already been described for alpaca (Vicugna pacos), but little data are available on success rate and the influence of diluent on it.
The aim was to investigate the effectiveness of epididymis incision as an extraction method.
Post-castration epididymides (n = 78) were incised and semen was collected from cut surfaces. Further sperm were flushed with two semen extenders. For the left epididymis, a diluent without animal proteins and for the right, a diluent with egg yolk was utilised. Collected sperms were immediately spermatologically examined.
Due to incorrect measurements, the samples of seven epididymides were not analysed. An evaluation was possible in 58 samples. Average density was 108.80 ± 83.28 million/mL and motility was 53.30 ± 18.17%. On average, 76.70 ± 11.60% of the sperm were vital in eosin-stained specimens. In the hypoosmotic swelling test, an average of 69.50 ± 10.48% of the sperm had an intact plasma membrane. Semen extender had no effect on spermatological parameters (p > 0.05). Overall success rate of sperm recovery was 83%. Extraction of epididymal sperm was possible in 68.80% of the 2-year-old males. The 13 epididymides from which no sperm recovery was possible were histologically examined and for 10, there was no histological evidence of sperm. The corrected success rate was 95.60%.
Testicular volume (p = 0.0453), but not age (p = 0.62), had an effect on the probability of obtaining sperm.
附睾精子可以在去势后或死后采集。这种方法已经在羊驼（Vicugna pacos）中得到描述，但关于成功率和稀释剂对其的影响的数据很少。
本研究旨在探讨附睾切开术作为一种提取方法的有效性。
对去势后的附睾（n=78）进行切开，并从切开的表面收集精液。进一步用两种精液稀释液冲洗精子。对于左侧附睾，使用不含动物蛋白的稀释液，对于右侧附睾，使用含蛋黄的稀释液。收集的精子立即进行精子学检查。
由于测量不正确，有 7 个附睾的样本未进行分析。在 58 个样本中进行了评估。平均密度为 108.80±83.28 百万/毫升，活力为 53.30±18.17%。平均有 76.70±11.60%的精子在伊红染色标本中存活。在低渗肿胀试验中，平均有 69.50±10.48%的精子具有完整的质膜。精液稀释液对精子学参数没有影响（p>0.05）。精子回收的总体成功率为 83%。在 2 岁的雄性中，有 68.80%可以提取附睾精子。在 13 个未提取到精子的附睾中，进行了组织学检查，其中 10 个附睾没有精子的组织学证据。校正后的成功率为 95.60%。
睾丸体积（p=0.0453）而不是年龄（p=0.62）对获得精子的概率有影响。
