Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden; Al-Nahrain University-High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Clinical Reproductive Physiology, Baghdad, Iraq.
Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden.
Anim Reprod Sci. 2020 Dec;223:106629. doi: 10.1016/j.anireprosci.2020.106629. Epub 2020 Oct 16.
Artificial insemination would be a useful technique for alpaca breeders to use as an aid to breeding to increase fleece quality. The technique, however, is not well developed in alpacas, partly because of the viscous nature of their seminal plasma. Castration conducted for husbandry purposes can provide a source of epididymal spermatozoa to test semen extenders or handling regimens, thus circumventing the problem of the viscous ejaculate. In this experiment, two semen extenders (Andromed and INRA96) developed for other species (bovine and equine, respectively) were tested with alpaca spermatozoa derived from the cauda epididymis. Sperm total motility (mean ± SEM A: 29.1 ± 4.8 % compared with I: 35.4 ± 4.8 %; NS), membrane integrity (A: 58 ± 9% compared with I: 56 ± 9%; NS) and acrosome integrity (A: 65 ± 7% compared with I: 54 ± 7%; NS) were not different between the two extenders. Progressive motility with use of INRA96 was greater after incubating for 30 min than after incubating for 10 min (35 ± 4% vs. 12 ± 4%, respectively; P = 0.03). In conclusion, viable epididymal spermatozoa could be extracted from the castrated organs after overnight transport. There were no differences in sperm quality between the two extenders; therefore, it appears that either extender could be used for alpaca spermatozoa. These results could help in the development of a technique for artificial insemination in alpacas.
人工授精将是一个有用的技术，为羊驼饲养者使用作为辅助繁殖，以提高羊毛质量。然而，该技术在羊驼中还没有得到很好的发展，部分原因是它们的精液等离子体粘性。为了畜牧业的目的进行的去势可以提供附睾精子的来源，以测试精液稀释剂或处理方案，从而避免粘性精液的问题。在这个实验中，两种为其他物种（牛和马，分别）开发的精液稀释剂（Andromed 和 INRA96）用来自羊驼尾部附睾的精子进行了测试。精子总活力（平均值±SEM A：29.1±4.8%，与 I：35.4±4.8%；无显著性差异）、膜完整性（A：58±9%，与 I：56±9%；无显著性差异）和顶体完整性（A：65±7%，与 I：54±7%；无显著性差异）在两种稀释剂之间没有差异。用 INRA96 孵育 30 分钟后的精子前向运动率大于孵育 10 分钟后的精子前向运动率（分别为 35±4%和 12±4%；P=0.03）。总之，在隔夜运输后，可以从去势的器官中提取出有活力的附睾精子。两种稀释剂之间的精子质量没有差异；因此，两种稀释剂都可以用于羊驼的精子。这些结果可以帮助开发羊驼人工授精技术。
