缺氧下人牙周膜干细胞 ceRNA 网络的综合分析。

Integrative Analysis of ceRNA Networks in human periodontal ligament stem cells under hypoxia.

机构信息

Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University & Department of Endodontic, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China.

Institute of Stomatology, Nanjing Medical University, Nanjing, China.

出版信息

Oral Dis. 2023 Apr;29(3):1197-1213. doi: 10.1111/odi.14096. Epub 2021 Dec 15.

DOI:10.1111/odi.14096

PMID:34874587

Abstract

OBJECTIVE

This study aims to investigate the regulatory effect of hypoxia on human periodontal ligament stem cells (PDLSCs) through RNA sequencing (RNA SEQ). Human PDLSCs were cultured in normoxia (20% O ) or hypoxia (2% O ).

MATERIAL AND METHODS

Total RNA was extracted and sequenced. The expression profiles of circRNAs, lncRNAs, and miRNAs were determined, and the lncRNA/circRNA-miRNA-mRNA networks were analyzed.

RESULTS

In total, 15 miRNAs, 449 lncRNAs, and 53 circRNAs were differentially expressed. Among them, 21 circRNAs, 262 lncRNAs, 5 miRNAs, and 5 mRNAs were selected to construct competing endogenous RNA (ceRNA) networks. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were carried out to explore potential related pathways and regulatory functions. Several ceRNA axes (lncRNA-FTX/circRNA-FAT1-hsa-miR-4781-3p-SMAD5 and circRNA LPAR1-hsa-miR-342-3p-ADAR) may provide a theoretical basis on the study of osteogenic differentiation of PDLSCs under hypoxia.

CONCLUSION

This study revealed that the expression profiles of circRNAs, lncRNAs, and miRNAs had changed significantly in PDLSCs cultured in 2% O ; specific circRNAs/lncRNAs may play a competitive role in the differentiation of PDLSCs.

摘要

目的

本研究通过 RNA 测序（RNA SEQ）探讨低氧对人牙周膜干细胞（PDLSCs）的调控作用。将人牙周膜干细胞在常氧（20% O ）或低氧（2% O ）下培养。

材料与方法

提取总 RNA 并进行测序。测定 circRNAs、lncRNAs 和 miRNAs 的表达谱，并分析 lncRNA/circRNA-miRNA-mRNA 网络。

结果

共鉴定出 15 个 miRNA、449 个 lncRNA 和 53 个 circRNA 差异表达。其中，选择了 21 个 circRNA、262 个 lncRNA、5 个 miRNA 和 5 个 mRNA 构建竞争性内源 RNA（ceRNA）网络。进行基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析，以探讨潜在的相关途径和调控功能。几个 ceRNA 轴（lncRNA-FTX/circRNA-FAT1-hsa-miR-4781-3p-SMAD5 和 circRNA LPAR1-hsa-miR-342-3p-ADAR）可能为低氧下人牙周膜干细胞成骨分化的研究提供理论基础。