Vos O, van der Schans G P, Roos-Verheij W S
Int J Radiat Biol Relat Stud Phys Chem Med. 1986 Jul;50(1):155-65. doi: 10.1080/09553008614550531.
The intracellular glutathione (GSH) content of HeLa, CHO and V79 cells was reduced by incubating the cells in growth medium containing buthionine sulphoximine or diethyl maleate (DEM). Clonogenicity, single-strand DNA breaks (ssb) and double-strand DNA breaks (dsb) were used as criteria for radiation-induced damage after X- or gamma-irradiation. In survival experiments, DEM gave a slightly larger sensitization although it gave a smaller reduction of the intracellular GSH. In general, sensitization was larger for dsb than for ssb, also the reduction of the o.e.r. was generally larger for dsb than for ssb. This may be due to the higher dose rate in case of dsb experiments resulting in a higher rate of radiochemical oxygen consumption. In general, no effect was found on post-irradiation repair of ssb and dsb.
通过将HeLa、CHO和V79细胞在含有丁硫氨酸亚砜胺或马来酸二乙酯（DEM）的生长培养基中培养，可降低细胞内谷胱甘肽（GSH）的含量。克隆形成能力、单链DNA断裂（ssb）和双链DNA断裂（dsb）被用作X射线或γ射线照射后辐射诱导损伤的标准。在存活实验中，尽管DEM使细胞内GSH的降低幅度较小，但它产生的敏化作用略大。一般来说，dsb的敏化作用比ssb大，o.e.r.（氧增强比）的降低通常也是dsb比ssb大。这可能是由于dsb实验中的剂量率较高，导致放射化学耗氧率较高。一般来说，未发现对ssb和dsb的照射后修复有影响。
