Shi Wei, Sun Shuge, Han Yu, Tang Yu, Zhou Weishang, Zhang Weixia, Du Xueying, Huang Lin, Liu Guangxu
College of Animal Sciences, Zhejiang University, Hangzhou, PR China.
College of Animal Sciences, Zhejiang University, Hangzhou, PR China.
Aquat Toxicol. 2022 Jan;242:106049. doi: 10.1016/j.aquatox.2021.106049. Epub 2021 Dec 1.
By employing external fertilization (broadcast spawning) as a mating strategy, the gametes and subsequent fertilization of various marine invertebrates are directly subjected to pollution. Although microplastics (MPs) are ubiquitous in marine environments, their potential effects on the fertilization of broadcast spawners remain largely unknown. Therefore in this study, the impacts of polystyrene MPs on the fertilization success of broadcast spawning bivalve (Tegillarca granosa) were investigated. In order to reveal the underlying mechanisms affecting fertilization, the sperm swimming performance, sperm ATP status, sperm viability, DNA integrity, gamete collision probability, gamete fusion efficiency, enzymatic antioxidants, and key ion transport enzyme activities were analyzed. The results showed that MPs weakened the sperm swimming performance through reducing ATP production and cell viability, thus leading to the decreased probability of gamete collision. Furthermore, MPs affected ion transport in the gametes by inducing oxidative stress, which resulted in gamete fusion failure. In conclusion, this study demonstrates that MPs could significantly decrease the fertilization success of T. granosa through reducing gamete collision and lowering gamete fusion efficiency.
通过采用体外受精（散播产卵）作为交配策略，各种海洋无脊椎动物的配子及随后的受精过程会直接受到污染影响。尽管微塑料在海洋环境中无处不在，但其对散播产卵生物受精的潜在影响仍 largely 未知。因此，在本研究中，我们调查了聚苯乙烯微塑料对散播产卵双壳贝类（泥蚶）受精成功率的影响。为了揭示影响受精的潜在机制，我们分析了精子游动性能、精子 ATP 状态、精子活力、DNA 完整性、配子碰撞概率、配子融合效率、酶促抗氧化剂以及关键离子转运酶活性。结果表明，微塑料通过降低 ATP 生成和细胞活力削弱了精子游动性能，从而导致配子碰撞概率降低。此外，微塑料通过诱导氧化应激影响配子中的离子转运，进而导致配子融合失败。总之，本研究表明微塑料可通过降低配子碰撞和配子融合效率显著降低泥蚶的受精成功率。
