Laboratory of Morpho-physiology and Biochemistry of Neotropical Fishes, Federal University of Tocantins (UFT), Araguaína, Tocantins, Brazil.
J Environ Sci Health B. 2021;56(12):1057-1065. doi: 10.1080/03601234.2021.2011555. Epub 2021 Dec 7.
Possible changes in the morphology of the gills and liver of after exposure to TCF were evaluated. The fish were distributed into five groups in triplicate (n = 10 in each group: 0.0; 0.125; 0.25; 0.5; 1.0 mg/L of the commercial product NEGUVON®, for 96 h. TCF induced a high histopathological index associated with circulatory disorders (congestion and aneurysm), as well as progressive changes (lamellar hyperplasia, capillary dilation, epithelial detachment and edema) at the highest concentrations (0.25; 0.5 and 1.0 mg/L). In addition to the histopathological disturbances, there was an increase in the levels of neutral glycoproteins and alterations in the histomorphometry of the secondary lamellae (total height, apical region, median, basal and interlamellar) at the highest concentrations. In the liver, the alterations were more intensified, in addition to circulatory (congestion) and regressive disturbances (loss of hepatocyte architecture and accumulation of intracellular substances), progressive alterations (focal necrosis) were observed, indicating a high degree of hepatic tissue involvement. The results indicate that the use of TCF concentrations above 0.25 mg/L promotes histopathological, histomorphometric and histochemical changes in the gills and liver of , imposing a biological risk that affects the aquatic environment and the health of fish.
评估了接触 TCF 后 鳃和肝脏形态的可能变化。将鱼分为五组，每组重复三次（每组 10 条：0.0；0.125；0.25；0.5；1.0 mg/L 的商用 NEGUVON®，暴露 96 小时。TCF 诱导了与循环障碍（充血和动脉瘤）相关的高组织病理学指数，以及在最高浓度（0.25；0.5 和 1.0 mg/L）下的渐进性变化（板层增生、毛细血管扩张、上皮脱落和水肿）。除了组织病理学紊乱外，在最高浓度下，中性糖蛋白的水平增加，并且次级板层的组织形态发生改变（总高度、顶端区域、中间、基底和板间）。在肝脏中，除了循环（充血）和退行性紊乱（肝细胞结构丧失和细胞内物质积累）外，还观察到进行性改变（局灶性坏死），表明肝组织受累程度较高。结果表明，使用 TCF 浓度超过 0.25 mg/L 会导致 和 的鳃和肝脏发生组织病理学、组织形态计量学和组织化学变化，对水生环境和鱼类健康造成生物风险。
