Silva Hallana Cristina Menezes da, Lobo Igor Kelvyn Cavalcante, Silva André Gentil da, Gomes Ana Lúcia Silva, Duncan Wallice Paxiúba, Silva Juliana Costa, Lopes Fabrício M, Artoni Roberto Ferreira, Matoso Daniele Aparecida
Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiva, Instituto Nacional de Pesquisas da Amazônia, Manaus 69067-375, AM, Brazil.
Programa de Pós-Graduação em Genética, Laboratório de Algoritmos em Biologia, Universidade Federal de Minas Gerais, Belo-Horizonte 31270-800, MG, Brazil.
Animals (Basel). 2025 Jun 19;15(12):1807. doi: 10.3390/ani15121807.
Trichlorfon is an antiparasitic agent widely used to control pests and parasites in farmed fish. Tambaqui () is the most commercially important characid species farmed in the Amazon region. Trichlorfon exposure is known to cause physiological damage in fish due to its organophosphate nature. In this study, we used RNA-Seq to investigate the hepatic response of tambaqui following exposure to 0.435 mg/L of trichlorfon. The analysis revealed activation of several metabolic pathways, particularly those related to tumor processes, immune responses, and apoptosis. Additionally, we identified upregulation of solute carrier (SLC) genes, which may facilitate trichlorfon entry into hepatocytes. These findings enhance our understanding of fish responses to antiparasitic agents and support further research into the molecular impacts of organophosphate compounds in aquaculture species.
敌百虫是一种广泛用于控制养殖鱼类病虫害的抗寄生虫剂。坦巴基鱼（）是亚马逊地区养殖的最重要的商业性脂鲤科鱼类品种。已知由于敌百虫的有机磷性质，其暴露会对鱼类造成生理损害。在本研究中，我们使用RNA测序来研究坦巴基鱼在暴露于0.435 mg/L敌百虫后的肝脏反应。分析揭示了几种代谢途径的激活，特别是那些与肿瘤过程、免疫反应和细胞凋亡相关的途径。此外，我们鉴定出溶质载体（SLC）基因的上调，这可能有助于敌百虫进入肝细胞。这些发现增进了我们对鱼类对抗寄生虫剂反应的理解，并支持对有机磷化合物在水产养殖物种中的分子影响进行进一步研究。
