• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

流式细胞术分析三叉神经痛大鼠模型中的免疫和神经胶质细胞。

Flow cytometry analysis of immune and glial cells in a trigeminal neuralgia rat model.

机构信息

Public Technology Service Center of Fujian Medical University; Laboratory of Clinical Applied Anatomy, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350122, P.R. China.

Key Laboratory of Brain Aging and Neurodegenerative Diseases of Fujian Province, Fuzhou, 350122, P.R. China.

出版信息

Sci Rep. 2021 Dec 7;11(1):23569. doi: 10.1038/s41598-021-02911-x.

DOI:10.1038/s41598-021-02911-x
PMID:34876649
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8651642/
Abstract

Microvascular compression of the trigeminal root entry zone (TREZ) is the main cause of most primary trigeminal neuralgia (TN), change of glial plasticity was previously studied in the TREZ of TN rat model induced by chronic compression. To better understand the role of astrocytes and immune cells in the TREZ, different cell markers including glial fibrillary acidic protein (GFAP), complement C3, S100A10, CD45, CD11b, glutamate-aspartate transporter (GLAST), Iba-1 and TMEM119 were used in the TN rat model by immunohistochemistry and flow cytometry. On the post operation day 28, GFAP/C3-positive A1 astrocytes and GFAP/S100A10-positive A2 astrocytes were activated in the TREZ after compression injury, there were no statistical differences in the ratios of A1/A2 astrocytes between the sham and TN groups. There was no significant difference in Iba-1-positive cells between the two groups. The ratios of infiltrating lymphocytes (CD45+CD11b-) (p = 0.0075) and infiltrating macrophages (CD45highCD11b+) (p = 0.0388) were significantly higher than those of the sham group. In conclusion, different subtypes A1/A2 astrocytes in the TREZ were activated after compression injury, infiltrating macrophages and lymphocytes increased, these neuroimmune cells in the TREZ may participate in the pathogenesis of TN rat model.

摘要

三叉神经根入口区（TREZ）微血管压迫是大多数原发性三叉神经痛（TN）的主要原因，此前在慢性压迫诱导的 TN 大鼠模型的 TREZ 中研究了神经胶质可塑性的变化。为了更好地了解星形胶质细胞和免疫细胞在 TREZ 中的作用，通过免疫组织化学和流式细胞术使用不同的细胞标志物，包括神经胶质纤维酸性蛋白（GFAP）、补体 C3、S100A10、CD45、CD11b、谷氨酸-天冬氨酸转运体（GLAST）、Iba-1 和 TMEM119，研究了 TN 大鼠模型。在手术后第 28 天，在压迫损伤后 TREZ 中激活了 GFAP/C3 阳性 A1 星形胶质细胞和 GFAP/S100A10 阳性 A2 星形胶质细胞，但 sham 和 TN 组之间 A1/A2 星形胶质细胞的比例没有统计学差异。两组之间的 Iba-1 阳性细胞没有显著差异。浸润淋巴细胞（CD45+CD11b-）（p=0.0075）和浸润巨噬细胞（CD45highCD11b+）（p=0.0388）的比例明显高于 sham 组。总之，在压迫损伤后 TREZ 中的不同亚型 A1/A2 星形胶质细胞被激活，浸润的巨噬细胞和淋巴细胞增加，这些 TREZ 中的神经免疫细胞可能参与了 TN 大鼠模型的发病机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd06/8651642/ff0ea09e0605/41598_2021_2911_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd06/8651642/ca3403163bc1/41598_2021_2911_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd06/8651642/dbe6427ceb4c/41598_2021_2911_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd06/8651642/e61c54d720d1/41598_2021_2911_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd06/8651642/819ed2e49b66/41598_2021_2911_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd06/8651642/e5bd90be8575/41598_2021_2911_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd06/8651642/d2f8a1bff489/41598_2021_2911_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd06/8651642/7287e66c7f75/41598_2021_2911_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd06/8651642/ff0ea09e0605/41598_2021_2911_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd06/8651642/ca3403163bc1/41598_2021_2911_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd06/8651642/dbe6427ceb4c/41598_2021_2911_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd06/8651642/e61c54d720d1/41598_2021_2911_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd06/8651642/819ed2e49b66/41598_2021_2911_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd06/8651642/e5bd90be8575/41598_2021_2911_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd06/8651642/d2f8a1bff489/41598_2021_2911_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd06/8651642/7287e66c7f75/41598_2021_2911_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd06/8651642/ff0ea09e0605/41598_2021_2911_Fig8_HTML.jpg

相似文献

1
Flow cytometry analysis of immune and glial cells in a trigeminal neuralgia rat model.流式细胞术分析三叉神经痛大鼠模型中的免疫和神经胶质细胞。
Sci Rep. 2021 Dec 7;11(1):23569. doi: 10.1038/s41598-021-02911-x.
2
Glial Plasticity in the Trigeminal Root Entry Zone of a Rat Trigeminal Neuralgia Animal Model.大鼠三叉神经痛动物模型三叉神经根进入区神经胶质可塑性研究。
Neurochem Res. 2019 Aug;44(8):1893-1902. doi: 10.1007/s11064-019-02824-2. Epub 2019 Jun 17.
3
An animal model for trigeminal neuralgia by compression of the trigeminal nerve root.神经根压迫致三叉神经痛动物模型
Pain Physician. 2012 Mar-Apr;15(2):187-96.
4
Immunohistochemical analysis of histone H3 acetylation in the trigeminal root entry zone in an animal model of trigeminal neuralgia.免疫组化分析三叉神经痛动物模型三叉神经根进入区组蛋白 H3 乙酰化。
J Neurosurg. 2018 Nov 9;131(3):828-838. doi: 10.3171/2018.5.JNS172948. Print 2019 Sep 1.
5
Brain-derived neurotrophic factor and Glial cell line-derived neurotrophic factor expressions in the trigeminal root entry zone and trigeminal ganglion neurons of a trigeminal neuralgia rat model.三叉神经痛大鼠模型三叉神经根进入区和三叉神经节神经元中脑源性神经营养因子和胶质细胞源性神经营养因子的表达。
Anat Rec (Hoboken). 2020 Dec;303(12):3014-3023. doi: 10.1002/ar.24364. Epub 2020 Jan 17.
6
Trigeminal injury causes kappa opioid-dependent allodynic, glial and immune cell responses in mice.三叉神经损伤导致小鼠κ阿片依赖的痛觉过敏、神经胶质和免疫细胞反应。
Mol Pain. 2010 Jan 29;6:8. doi: 10.1186/1744-8069-6-8.
7
Using RNA-Seq to Explore the Hub Genes in the Trigeminal Root Entry Zone of Rats by Compression Injury.利用 RNA-Seq 技术探索压迫性损伤大鼠三叉神经根进入区的枢纽基因。
Pain Physician. 2021 Aug;24(5):E573-E581.
8
Activation of A1 reactive astrocytes in the medullary dorsal horn of rats participates in the chronification of trigeminal neuralgia.大鼠延髓背角 A1 反应性星形胶质细胞的激活参与了三叉神经痛的慢性化。
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2024 Jan 28;49(1):21-28. doi: 10.11817/j.issn.1672-7347.2024.230303.
9
Blood-nerve barrier disruption and coagulation system activation induced by mechanical compression injury participate in the peripheral sensitization of trigeminal neuralgia.机械压迫损伤所致的血神经屏障破坏和凝血系统激活参与了三叉神经痛的外周敏化。
Front Mol Neurosci. 2022 Dec 20;15:1059980. doi: 10.3389/fnmol.2022.1059980. eCollection 2022.
10
Atrophic changes in the trigeminal nerves of patients with trigeminal neuralgia due to neurovascular compression and their association with the severity of compression and clinical outcomes.神经血管压迫所致三叉神经痛患者三叉神经的萎缩性改变及其与压迫严重程度和临床结局的关联。
J Neurosurg. 2014 Jun;120(6):1484-95. doi: 10.3171/2014.2.JNS131288. Epub 2014 Apr 18.

引用本文的文献

1
Genetic influence of the brain imaging phenotypes, brain and cerebrospinal fluid metabolites and brain genes on migraine subtypes: a Mendelian randomization and multi-omics study.脑成像表型、脑和脑脊液代谢物以及脑基因对偏头痛亚型的遗传影响：一项孟德尔随机化和多组学研究。
J Headache Pain. 2025 May 20;26(1):124. doi: 10.1186/s10194-025-02063-7.
2
Investigating the causal impact of gut microbiota on trigeminal neuralgia: a bidirectional Mendelian randomization study.探究肠道微生物群对三叉神经痛的因果影响：一项双向孟德尔随机化研究。
Front Microbiol. 2025 Feb 27;16:1420978. doi: 10.3389/fmicb.2025.1420978. eCollection 2025.
3

本文引用的文献

1
Glial Cells Promote Myelin Formation and Elimination.神经胶质细胞促进髓鞘形成与清除。
Front Cell Dev Biol. 2021 May 11;9:661486. doi: 10.3389/fcell.2021.661486. eCollection 2021.
2
RIPK1 activation mediates neuroinflammation and disease progression in multiple sclerosis.RIPK1 激活介导多发性硬化症中的神经炎症和疾病进展。
Cell Rep. 2021 May 11;35(6):109112. doi: 10.1016/j.celrep.2021.109112.
3
Can quantifying morphology and TMEM119 expression distinguish between microglia and infiltrating macrophages after ischemic stroke and reperfusion in male and female mice?
Compression Degree of Trigeminal Nerve and Type of Conflicting Vessels Determine Short- and Long-Term Complete Pain Relief in Adult Patients with Primary Trigeminal Neuralgia After Microvascular Decompression: A Three-Year Retrospective Study of 200 Adult Patients with Primary Trigeminal Neuralgia.
三叉神经压迫程度和冲突血管类型决定原发性三叉神经痛成年患者微血管减压术后的短期和长期完全疼痛缓解：一项对200例原发性三叉神经痛成年患者的三年回顾性研究
J Pain Res. 2023 Dec 7;16:4191-4207. doi: 10.2147/JPR.S429713. eCollection 2023.
4
A comprehensive two-sample Mendelian randomization analysis of trigeminal neuralgia and modifiable risk factors.三叉神经痛与可改变风险因素的综合两样本孟德尔随机化分析。
Front Neurol. 2023 Nov 22;14:1292958. doi: 10.3389/fneur.2023.1292958. eCollection 2023.
5
Interferon-γ controls aquaporin 4-specific Th17 and B cells in neuromyelitis optica spectrum disorder.干扰素-γ 调控视神经脊髓炎谱系疾病中水通道蛋白 4 特异性 Th17 和 B 细胞。
Brain. 2024 Apr 4;147(4):1344-1361. doi: 10.1093/brain/awad373.
6
Preclinical orofacial pain assays and measures and chronic primary orofacial pain research: where we are and where we need to go.临床前口腔面部疼痛检测与测量以及慢性原发性口腔面部疼痛研究：我们所处的位置与需要前进的方向。
Front Pain Res (Lausanne). 2023 May 24;4:1150749. doi: 10.3389/fpain.2023.1150749. eCollection 2023.
在雄性和雌性小鼠缺血性中风及再灌注后，量化形态学和TMEM119表达能否区分小胶质细胞和浸润性巨噬细胞？
J Neuroinflammation. 2021 Feb 22;18(1):58. doi: 10.1186/s12974-021-02105-2.
4
Astrocyte Crosstalk in CNS Inflammation.星形胶质细胞在中枢神经系统炎症中的相互作用。
Neuron. 2020 Nov 25;108(4):608-622. doi: 10.1016/j.neuron.2020.08.012. Epub 2020 Sep 7.
5
The Increased Densities, But Different Distributions, of Both C3 and S100A10 Immunopositive Astrocyte-Like Cells in Alzheimer's Disease Brains Suggest Possible Roles for Both A1 and A2 Astrocytes in the Disease Pathogenesis.阿尔茨海默病大脑中C3和S100A10免疫阳性星形胶质细胞样细胞密度增加但分布不同，提示A1和A2星形胶质细胞在疾病发病机制中可能发挥作用。
Brain Sci. 2020 Jul 31;10(8):503. doi: 10.3390/brainsci10080503.
6
Brain-derived neurotrophic factor and Glial cell line-derived neurotrophic factor expressions in the trigeminal root entry zone and trigeminal ganglion neurons of a trigeminal neuralgia rat model.三叉神经痛大鼠模型三叉神经根进入区和三叉神经节神经元中脑源性神经营养因子和胶质细胞源性神经营养因子的表达。
Anat Rec (Hoboken). 2020 Dec;303(12):3014-3023. doi: 10.1002/ar.24364. Epub 2020 Jan 17.
7
Occurrence of Transmembrane Protein 119 in the Retina is Not Restricted to the Microglia: An Immunohistochemical Study.视网膜中跨膜蛋白119的表达并不局限于小胶质细胞：一项免疫组织化学研究。
Transl Vis Sci Technol. 2019 Dec 12;8(6):29. doi: 10.1167/tvst.8.6.29. eCollection 2019 Nov.
8
Early postnatal manganese exposure causes arousal dysregulation and lasting hypofunctioning of the prefrontal cortex catecholaminergic systems.早期产后锰暴露导致觉醒调节障碍和前额叶皮质儿茶酚胺能系统持续功能低下。
J Neurochem. 2020 Jun;153(5):631-649. doi: 10.1111/jnc.14934. Epub 2020 Jan 10.
9
Role of macrophages and activated microglia in neuropathic pain associated with chronic progressive spinal cord compression.巨噬细胞和激活的小胶质细胞在慢性进行性脊髓压迫相关神经性疼痛中的作用。
Sci Rep. 2019 Oct 30;9(1):15656. doi: 10.1038/s41598-019-52234-1.
10
The astrocyte transcriptome in EAE optic neuritis shows complement activation and reveals a sex difference in astrocytic C3 expression.实验性自身免疫性脑脊髓炎视神经炎中天麻细胞转录组显示补体激活，并揭示了星形胶质细胞 C3 表达的性别差异。
Sci Rep. 2019 Jul 10;9(1):10010. doi: 10.1038/s41598-019-46232-6.