Vietti Ramus G, Cesano L, Barbalonga A, Pallisco O
Minerva Med. 1986 May 19;77(21):917-22.
Lidocaine and Verapamil at pharmacological doses which for single drug are not cytotoxic, when used together in vitro, inhibit DNA replication in PHA-stimulated lymphocytes but not in Jurkat cell (T-ALL line) cultures. At the same concentration the two drugs used in association with very low doses of Vincristine are cytotoxic to PHA-stimulated lymphocytes and Jurkat cells. Cytotoxic action of Doxorubicin is not increased by Lidocaine or by Verapamil or by an association of the two drugs. Changes in calcium ion concentration in the medium did not show any significant effect. These results suggest that Lidocaine and Verapamil have a common mechanism of action and have a toxic action on the same cell structure of Vincristine; furthermore the cytotoxic action of Vincristine is considerably increased. These in vitro effects could be tested in animal models.
利多卡因和维拉帕米在药理剂量下（单用时对细胞无毒性），体外联合使用时可抑制PHA刺激的淋巴细胞中的DNA复制，但对Jurkat细胞（T-ALL系）培养物无此作用。在相同浓度下，这两种药物与极低剂量的长春新碱联合使用时，对PHA刺激的淋巴细胞和Jurkat细胞具有细胞毒性。阿霉素的细胞毒性作用不会因利多卡因或维拉帕米或两种药物联合使用而增强。培养基中钙离子浓度的变化未显示出任何显著影响。这些结果表明，利多卡因和维拉帕米具有共同的作用机制，对长春新碱的相同细胞结构具有毒性作用；此外，长春新碱的细胞毒性作用显著增强。这些体外效应可在动物模型中进行测试。
