College of Animal Science and Technology, Northwest A&F University, Yangling, China.
National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan.
Anim Biotechnol. 2023 Nov;34(4):1050-1057. doi: 10.1080/10495398.2021.2011305. Epub 2021 Dec 8.
Heat stress affects the animal production and causes serious economic losses to the husbandry. Tectonin beta-propeller repeat containing 2 () gene plays an important role in autophagy which may affect the temperature sensation in animals. A missense mutation (XM_024981840.1:c.3989 G > A p.Arg1330His) of the transcripts X4 in the bovine gene was identified. In this study, the c.3989 G > A variant in gene was genotyped in a total of 25 cattle breeds (520 individuals). Our results indicated that the frequency of A allele showed a decreasing pattern from southern cattle to northern cattle, while the frequency of G allele showed the opposite pattern, which was consistent with the climate distribution of China. Compared with the GG genotype, southern cattle carried more the AA and AG genotypes. Furthermore, the association results carried out that the frequencies of genotypes (GG, AG, AA) and the value of climate parameters (mean annual temperature (T), relative humidity (RH) and temperature humidity index (THI) were significantly correlated ( < 0.01). Hence, we speculated that the c.3989 G > A variant of gene was associated with the heat tolerance trait in Chinese cattle and the locus may be considered as a molecular marker for Chinese cattle breeding.
热应激会影响动物生产，并给畜牧业造成严重的经济损失。Tectonin beta-propeller repeat containing 2()基因在自噬中起着重要作用，它可能会影响动物的温度感知。在牛的基因中，发现了转录物 X4 的一个错义突变（XM_024981840.1:c.3989 G > A p.Arg1330His）。在这项研究中，总共在 25 个牛品种（520 个个体）中对基因中的 c.3989 G > A 变体进行了基因分型。我们的结果表明，A 等位基因的频率从南方牛到北方牛呈下降趋势，而 G 等位基因的频率则呈相反的趋势，这与中国的气候分布一致。与 GG 基因型相比，南方牛携带更多的 AA 和 AG 基因型。此外，关联结果表明，基因型（GG、AG、AA）的频率和气候参数（年平均温度（T）、相对湿度（RH）和温度湿度指数（THI）的值存在显著相关性（<0.01）。因此，我们推测基因中的 c.3989 G > A 变体与中国牛的耐热特性有关，该位点可被视为中国牛选育的分子标记。
