College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
State Key Laboratory of Veterinary Biotechnology, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China.
Curr Microbiol. 2021 Dec 4;79(1):1. doi: 10.1007/s00284-021-02697-1.
Despites Providencia heimbachae has been isolated from human, penguin, and bovine fetus, relatively little information is available regarding the pathogenicity and biologic characteristics of P. heimbachae. Here, we report that investigation of post-weaning diarrhea yielded bacterial isolates identified as P. heimbachae based on the biochemical tests and 16S ribosomal DNA sequence analysis. The two isolates were positive for utilization of Malonate, no gas production from glucose, and non-fermentation of D-mannitol, D-Galactose, and L-Rhamnose that were different from those of the type strain, and both of them have the ability of adhesion and invasion to IPEC-J2 cells, and were resistant to 21 out of the 41 antibiotics tested. In addition, the isolate 99101 was highly pathogenic to mice and piglets. Histopathology studies on nerve tissue of piglets that developed hindlimb paralysis showed microglia cell infiltration and neuron damage in the spinal cord. Notably, the strains could grow under low temperature (4 °C), which raise attention of a new risk factor for food safety. To the best of our knowledge, this is the first report of P. heimbachae strain caused post-weaning diarrhea in piglets in both natural and experimental conditions. These findings extended the knowledge of P. heimbachae as an important zoonotic agent, which should be given more attention during surveillance and diagnostics.
尽管 Providencia heimbachae 已从人类、企鹅和牛胎儿中分离出来，但关于其致病性和生物学特性的信息相对较少。在这里，我们报告了一项关于断奶后腹泻的调查结果，从该调查中分离出的细菌被鉴定为 P. heimbachae，这是基于生化试验和 16S 核糖体 DNA 序列分析得出的。这两个分离株均能利用丙二酸盐，但不能从葡萄糖中产生气体，不能发酵 D-甘露醇、D-半乳糖和 L-鼠李糖，这与模式株不同。此外，它们都具有黏附和侵袭 IPEC-J2 细胞的能力，并且对 41 种测试抗生素中的 21 种具有耐药性。此外，分离株 99101 对小鼠和仔猪具有高度致病性。对出现后肢瘫痪的仔猪神经组织进行的组织病理学研究显示，脊髓中的小胶质细胞浸润和神经元损伤。值得注意的是，这些菌株能够在低温（4°C）下生长，这引起了人们对食品安全新风险因素的关注。据我们所知，这是首次在自然和实验条件下报告 P. heimbachae 菌株引起仔猪断奶后腹泻。这些发现扩展了 P. heimbachae 作为一种重要的人畜共患病原体的知识，在监测和诊断中应给予更多关注。
