piRNAs 在促进和预防 C. elegans 基因沉默中的双重作用。
Dual roles for piRNAs in promoting and preventing gene silencing in C. elegans.
机构信息
Department of Biology, Colorado State University, Fort Collins, CO 80523, USA.
Department of Biology, Colorado State University, Fort Collins, CO 80523, USA; Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523, USA.
出版信息
Cell Rep. 2021 Dec 7;37(10):110101. doi: 10.1016/j.celrep.2021.110101.
Piwi-interacting RNAs (piRNAs) regulate many biological processes through mechanisms that are not fully understood. In Caenorhabditis elegans, piRNAs intersect the endogenous RNA interference (RNAi) pathway, involving a distinct class of small RNAs called 22G-RNAs, to regulate gene expression in the germline. In the absence of piRNAs, 22G-RNA production from many genes is reduced, pointing to a role for piRNAs in facilitating endogenous RNAi. Here, however, we show that many genes gain, rather than lose, 22G-RNAs in the absence of piRNAs, which is in some instances coincident with RNA silencing. Aberrant 22G-RNA production is somewhat stochastic but once established can occur within a population for at least 50 generations. Thus, piRNAs both promote and suppress 22G-RNA production and gene silencing. rRNAs and histones are hypersusceptible to aberrant silencing, but we do not find evidence that their misexpression is the primary cause of the transgenerational sterility observed in piRNA-defective mutants.
Piwi 相互作用 RNA（piRNAs）通过尚未完全阐明的机制调控许多生物学过程。在秀丽隐杆线虫中，piRNAs 与内源性 RNA 干扰（RNAi）途径交叉，涉及一类称为 22G-RNAs 的独特小 RNA，以调控生殖系中的基因表达。在没有 piRNAs 的情况下，许多基因的 22G-RNA 产生减少，表明 piRNAs 在促进内源性 RNAi 中起作用。然而，在这里，我们表明，许多基因在没有 piRNAs 的情况下获得而不是失去 22G-RNAs，在某些情况下与 RNA 沉默同时发生。异常的 22G-RNA 产生有些随机，但一旦建立，至少可以在 50 代的种群中发生。因此，piRNAs 既促进又抑制 22G-RNA 的产生和基因沉默。rRNA 和组蛋白极易受到异常沉默的影响，但我们没有发现证据表明它们的错误表达是 piRNA 缺陷突变体中观察到的跨代不育的主要原因。
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