Qingdao Agricultural University, Qingdao, Shandong, 266109, China.
College of Agronomy, Liaocheng University, Liaocheng, Shandong, 252059, China; Shandong Donkey Industry Technology Collaborative Innovation Center, Liaocheng, Shandong, 252059, China.
Theriogenology. 2022 Feb;179:162-176. doi: 10.1016/j.theriogenology.2021.11.021. Epub 2021 Dec 2.
Deoxynivalenol (DON) and zearalenone (ZEA), which are commonly found in feed products, exhibit serious negative effects on the reproductive systems of domestic animals. However, the toxicity of mycotoxins on the uterine function of donkey (Equus asinus) remains unclear. This study investigated the biological effects of DON and ZEA exposure on donkey endometrial epithelial cells (EECs). It was administered 10 μM and 30 μM DON and ZEA to cells cultured in vitro. The results showed that 10 μM DON exposure markedly changed the expression levels of pyroptosis-associated genes and that 30 μM ZEA exposure changed the expression levels of inflammation-associated genes in EECs. The mRNA expression of cancer-promoting genes was markedly upregulated in cells exposed to DON and 30 μM ZEA; in particular, 10 μM and 30 μM DON and ZEA markedly disturbed the expression of androgen and estrogen secretion-related genes. Furthermore, Q-PCR, Western blot, and immunofluorescence analyses verified the different expression patterns of related genes in DON- and ZEA-exposed EECs. Collectively, these results illustrated the impact of exposure to different toxins and concrete toxicity on the mRNA expression of EECs from donkey in vitro.
脱氧雪腐镰刀菌烯醇（DON）和玉米赤霉烯酮（ZEA）是常见于饲料产品中的两种霉菌毒素，它们对家畜的生殖系统具有严重的负面影响。然而，霉菌毒素对驴（Equus asinus）子宫功能的毒性仍不清楚。本研究探讨了 DON 和 ZEA 暴露对驴子宫内膜上皮细胞（EEC）的生物学效应。将 10 μM 和 30 μM 的 DON 和 ZEA 施用于体外培养的细胞。结果表明，10 μM 的 DON 暴露显著改变了细胞中与细胞焦亡相关基因的表达水平，而 30 μM 的 ZEA 暴露则改变了与炎症相关基因的表达水平。暴露于 DON 和 30 μM ZEA 的细胞中促进癌症的基因的 mRNA 表达显著上调；特别是，10 μM 和 30 μM 的 DON 和 ZEA 明显干扰了雄激素和雌激素分泌相关基因的表达。此外，Q-PCR、Western blot 和免疫荧光分析验证了 DON 和 ZEA 暴露的 EECs 中相关基因的不同表达模式。总之，这些结果说明了不同毒素和具体毒性对体外驴 EECs 的 mRNA 表达的影响。
