Baqerkhani Mohammadreza, Soleimanzadeh Ali, Mohammadi Rahim
Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, P.O. Box: 57561-51818, Urmia, Iran.
Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
BMC Vet Res. 2024 Mar 11;20(1):99. doi: 10.1186/s12917-024-03915-1.
The aim of the present study was to examine donkey sperm quality after intratesticular injection of hypertonic mannitol (HM) and saline (HS).
Randomly assigned to five treatment groups were 15 adult male donkeys: (1) Control group (no treatment), (2) Surgery group (surgical castration for testosterone control), (3) NS group (normal saline intratesticular injection), (4) HS group (hypertonic saline), and (5) HM group. We injected 20 mL per testicle. We took 5 mL blood from all donkeys before injection. Castration was performed under general anesthesia 60 days later. Samples included blood and testicular tissue. Total motility (TM), progressive motility (PM), movementy features, DNA damage, morphology, viability, and plasma membrane functionality were evaluated. Hormone analyses, histomorphometric studies and oxidative stress indices including total antioxidant capacity (TAC), glutathione peroxidase (GPx), glutathione (GSH), superoxide dismutase (SOD), malondialdehyde (MDA), and NADP+/NADPH were evaluated. Apoptosis, pyroptosis-related Bax, Caspase-1, GSDMD, and Bcl-2 expression were also assessed.
In HS and HM groups, testosterone, epididymal sperm count, motility, viability, and plasma membrane functionality dropped while sperm DNA damage increased. HS and HM groups had significantly lower histomorphometric parameters, TAC, GPx, SOD, GSH, and Bcl-2 gene expression. MDA, NADP/NADPH, Bax, Caspase-1, and GSDMD gene expression were substantially higher in the HS and HM groups than in the control group.
Toxic effects of hypertonic saline and mannitol on reproductive parameters were seen following, hence, they might be considered as a good chemical sterilizing treatment in donkeys.
本研究旨在检测经睾丸内注射高渗甘露醇（HM）和生理盐水（HS）后驴的精子质量。
将15头成年雄性驴随机分为五个治疗组：（1）对照组（未治疗），（2）手术组（手术去势以控制睾酮），（3）生理盐水组（睾丸内注射生理盐水），（4）高渗盐水组（HS），和（5）高渗甘露醇组（HM）。每个睾丸注射20 mL。注射前从所有驴采集5 mL血液。60天后在全身麻醉下进行去势。样本包括血液和睾丸组织。评估了总活力（TM）、渐进活力（PM）、运动特征、DNA损伤、形态、活力和质膜功能。评估了激素分析、组织形态计量学研究以及氧化应激指标，包括总抗氧化能力（TAC）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、丙二醛（MDA）和NADP⁺/NADPH。还评估了凋亡、焦亡相关的Bax、Caspase-1、GSDMD和Bcl-2表达。
在HS组和HM组中，睾酮、附睾精子计数、活力、活力和质膜功能下降，而精子DNA损伤增加。HS组和HM组的组织形态计量学参数、TAC、GPx、SOD、GSH和Bcl-2基因表达显著降低。HS组和HM组的MDA、NADP/NADPH、Bax、Caspase-1和GSDMD基因表达明显高于对照组。
高渗盐水和甘露醇对生殖参数有毒性作用，因此，它们可能被认为是一种良好的驴化学绝育治疗方法。
