Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, College of Environmental Science and Engineering, Guilin 541004, Guangxi, China; Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area, Guilin University of Technology, Guilin 541004, China; National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agroenvironmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou 510650, China.
Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, College of Environmental Science and Engineering, Guilin 541004, Guangxi, China.
J Hazard Mater. 2022 Feb 15;424(Pt B):127545. doi: 10.1016/j.jhazmat.2021.127545. Epub 2021 Oct 19.
In this study, Aspergillus sydowii FJH-1 isolated from soil was verified to be a novel triphenyl phosphate (TPhP) degrader. Biodegradation efficiency of TPhP by Aspergillus sydowii FJH-1 exceeded 90% within 6 days under the optimal conditions (pH 4-9, 30 ℃, initial concentration less than 20 mg/L). Proteomics analysis uncovered the proteins perhaps involved in hydrolysis, hydroxylation, methylation and sulfonation of TPhP and the primary intracellular adaptive responses of Aspergillus sydowii FJH-1 to TPhP stress. The expression of carboxylic ester hydrolase along with several thioredoxin- and glutathione-dependent oxidoreductases were induced to withstand the toxicity of TPhP. The presence of TPhP also caused obvious upregulation of proteins concerned with glycolysis, pentose phosphate pathway and tricarboxylic acid cycle. Data from toxicological tests confirmed that the cytotoxicity and phytotoxicity of TPhP was effectively decreased after treatment with Aspergillus sydowii FJH-1. Additionally, bioaugmentation with Aspergillus sydowii FJH-1 was available for promoting TPhP removal in real water and water-sediment system. Collectively, the present study offered a deeper insight into the biodegradation mechanism and pathway of TPhP by a newly screened fungal strain Aspergillus sydowii FJH-1 and validated the feasibility of applying this novel degrader in the bioremediation of TPhP-polluted matrices.
在这项研究中，从土壤中分离出的枝顶孢 FJH-1 被证实是一种新型的磷酸三苯酯（TPhP）降解菌。在最佳条件下（pH4-9，30℃，初始浓度小于 20mg/L），枝顶孢 FJH-1 可在 6 天内将 TPhP 的生物降解效率超过 90%。蛋白质组学分析揭示了可能参与 TPhP 水解、羟化、甲基化和磺化的蛋白质，以及枝顶孢 FJH-1 对 TPhP 胁迫的主要细胞内适应反应。羧酸酯水解酶以及几种硫氧还蛋白和谷胱甘肽依赖的氧化还原酶的表达被诱导以抵御 TPhP 的毒性。TPhP 的存在还导致与糖酵解、戊糖磷酸途径和三羧酸循环相关的蛋白质明显上调。毒理学测试数据证实，用枝顶孢 FJH-1 处理后，TPhP 的细胞毒性和植物毒性有效降低。此外，用枝顶孢 FJH-1 进行生物强化可促进真实水体和水-沉积物系统中 TPhP 的去除。总之，本研究深入了解了新筛选的真菌菌株枝顶孢 FJH-1 对 TPhP 的生物降解机制和途径，并验证了该新型降解菌在 TPhP 污染基质生物修复中的可行性。
