Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, College of Environmental Science and Engineering, Guilin 541004, Guangxi, China; National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou 510650, China.
Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, College of Environmental Science and Engineering, Guilin 541004, Guangxi, China.
J Hazard Mater. 2021 Sep 5;417:125983. doi: 10.1016/j.jhazmat.2021.125983. Epub 2021 May 4.
So far, no information about the biodegradability of TPhP by white rot fungi has previously been made available, herein, Pycnoporus sanguineus was used as the representative to investigate the potential of white rot fungi in TPhP bioremediation. The results suggested that the biodegradation efficiency of 5 mg/L TPhP by P. sanguineus was 62.84% when pH was adjusted to 6 and initial glucose concentration was 5 g/L. Seven biodegradation products were identified, indicating that TPhP was biotransformed through oxidative cleavage, hydroxylation and methylation. The proteomic analysis revealed that cytochrome P450s, aromatic compound dioxygenase, oxidizing species-generating enzymes, methyltransferases and MFS general substrate transporters might occupy important roles in TPhP biotransformation. Carboxylesterase and glutathione S-transferase were induced to resist TPhP stress. The biotreatment by P. sanguineus contributed to a remarkable decrease of TPhP biotoxicity. Bioaugmentation with P. sanguineus could efficiently promote TPhP biodegradation in the water-sediment system due to the cooperation between P. sanguineus and some putative indigenous degraders, including Sphingobium, Burkholderia, Mycobacterium and Methylobacterium. Overall, this study provided the first insights into the degradation pathway, mechanism and security risk assessment of TPhP biodegradation by P. sanguineus and verified the feasibility of utilizing this fungus for TPhP bioremediation applications.
到目前为止，还没有关于白腐真菌对 TPhP 生物降解性的信息。在这里，使用红色射脉菌作为代表来研究白腐真菌在 TPhP 生物修复中的潜力。结果表明，当 pH 值调整为 6 且初始葡萄糖浓度为 5 g/L 时，红色射脉菌对 5mg/L TPhP 的生物降解效率为 62.84%。鉴定出 7 种生物降解产物，表明 TPhP 通过氧化裂解、羟化和甲基化进行生物转化。蛋白质组学分析表明，细胞色素 P450s、芳香族化合物双加氧酶、氧化物质生成酶、甲基转移酶和 MFS 一般底物转运蛋白可能在 TPhP 生物转化中占据重要地位。羧酸酯酶和谷胱甘肽 S-转移酶被诱导以抵抗 TPhP 胁迫。红色射脉菌的生物处理有助于显著降低 TPhP 的生物毒性。由于红色射脉菌与一些假定的土著降解菌（包括鞘氨醇单胞菌、伯克霍尔德菌、分枝杆菌和甲基杆菌）之间的合作，生物增强作用可有效地促进水-沉积物系统中 TPhP 的生物降解。总的来说，这项研究首次深入了解了红色射脉菌对 TPhP 的降解途径、机制和安全性评估，并验证了利用该真菌进行 TPhP 生物修复应用的可行性。
