FUSARIUM-ID v.3.0: An Updated, Downloadable Resource for Species Identification.

Species within are of global agricultural, medical, and food/feed safety concern and have been extensively characterized. However, accurate identification of species is challenging and usually requires DNA sequence data. -ID (http://isolate.fusariumdb.org/blast.php) is a publicly available database designed to support the identification of species using sequences of multiple phylogenetically informative loci, especially the highly informative ∼680-bp 5' portion of the translation elongation factor 1-alpha () gene that has been adopted as the primary barcoding locus in the genus. However, -ID v.1.0 and 2.0 had several limitations, including inconsistent metadata annotation for the archived sequences and poor representation of some species complexes and marker loci. Here, we present -ID v.3.0, which provides the following improvements: (i) additional and updated annotation of metadata for isolates associated with each sequence, (ii) expanded taxon representation in the sequence database, (iii) availability of the sequence database as a downloadable file to enable local BLAST queries, and (iv) a tutorial file for users to perform local BLAST searches using either freely available software, such as SequenceServer, BLAST+ executable in the command line, and Galaxy, or the proprietary Geneious software. -ID will be updated on a regular basis by archiving sequences of and other loci from newly identified species and greater in-depth sampling of currently recognized species.