Department of Obstetrics, ShuLan (Hangzhou) Hospital Affiliated to Zhejiang Shuren University Shulan International Medical College, Hangzhou City, Zhejiang, China.
Department of Medical Affairs, Hospital of Obstetrics and Gynecology Affiliated to Medical College of Zhejiang University, Hangzhou City, Zhejiang, China.
Endocr Metab Immune Disord Drug Targets. 2022;22(6):612-619. doi: 10.2174/1871530321666211208152709.
Preeclampsia (PE) is defined as a salient complication of late pregnancy. microRNAs (miRNAs) have emerged as critical biological regulators in PE. This study determined miR-27b-3p expression in serum of PE patients and investigated its clinical significance in PE.
This study enrolled a total of 130 pregnant women, including 90 PE patients (51 mild PE and 39 severe PE) and 40 healthy controls. miR-27b-3p expression in the serum of PE patients and healthy controls was detected using RT-qPCR. The correlation among miR-27b-3p expression and 24-h urine protein, systolic blood pressure (SBP), diastolic blood pressure (DBP), serum creatinine, and fetal birth weight was analyzed using Pearson's correlation coefficient. The targeting relationship between miR-27b-3p and PPARG was verified. PPARG protein level in PE patients was detected using ELISA kits. The predictive efficiency of miR-27b-3p and PPARG in PE was analyzed using the receiver operating characteristic (ROC) curve.
Compared to normal pregnant women, PE pregnant women, especially severe PE patients, had higher miR-27b-3p expression. miR-27b-3p was positively correlated with 24-h urine protein, SBP, DBP, and serum creatinine but negatively correlated with fetal birth weight. PPARG was poorly expressed in PE patients and negatively correlated with miR-27b-3p. ROC curve showed that both miR-27b-3p and PPARG had good predictive efficacy on PE.
miR-27b-3p expression in serum of pregnant women with PE was positively correlated with the severity of PE symptoms, suggesting the involvement of miR-27b-3p in PE occurrence.
子痫前期（PE）被定义为妊娠晚期的一种显著并发症。微小 RNA（miRNA）已成为 PE 中的关键生物学调节因子。本研究旨在确定 PE 患者血清中 miR-27b-3p 的表达，并探讨其在 PE 中的临床意义。
本研究共纳入 130 名孕妇，包括 90 名 PE 患者（51 名轻度 PE 和 39 名重度 PE）和 40 名健康对照。采用 RT-qPCR 检测 PE 患者和健康对照者血清中 miR-27b-3p 的表达。采用 Pearson 相关系数分析 miR-27b-3p 表达与 24 小时尿蛋白、收缩压（SBP）、舒张压（DBP）、血清肌酐和胎儿出生体重的相关性。验证 miR-27b-3p 与 PPARG 的靶向关系。采用 ELISA 试剂盒检测 PE 患者的 PPARG 蛋白水平。采用受试者工作特征（ROC）曲线分析 miR-27b-3p 和 PPARG 对 PE 的预测效率。
与正常孕妇相比，PE 孕妇，尤其是重度 PE 患者，miR-27b-3p 表达水平较高。miR-27b-3p 与 24 小时尿蛋白、SBP、DBP 和血清肌酐呈正相关，与胎儿出生体重呈负相关。PE 患者 PPARG 表达水平较低，与 miR-27b-3p 呈负相关。ROC 曲线表明，miR-27b-3p 和 PPARG 对 PE 均具有良好的预测效能。
PE 孕妇血清中 miR-27b-3p 的表达与 PE 症状的严重程度呈正相关，提示 miR-27b-3p 参与了 PE 的发生。
