Dong Na, Li Duanfei, Cai Huilan, Shi Ling, Huang Lu
Department of Obstetrics and Gynecology, Xianning Central Hospital, The First Affiliated Hospital of Hubei University of Science and Technology, No. 228 Jingui Road, Xian'an District, Xianning City, Hubei Province, 437100, China.
Department of Anesthesiology, Xianning Central Hospital, The First Affiliated Hospital of Hubei University of Science and Technology, No. 228 Jingui Road, Xian'an District, Xianning City, Hubei Province, 437100, China.
J Gynecol Obstet Hum Reprod. 2022 May;51(5):102357. doi: 10.1016/j.jogoh.2022.102357. Epub 2022 Mar 15.
Preeclampsia (PE) represents a salient complication of late pregnancy. Long noncoding RNAs (LncRNAs) are critical biological regulators in PE. This study investigated lncRNA MIR193BHG expression and clinical significance in PE.
Serum samples were collected from 116 PE patients, including 62 cases of mild PE (mPE) and 54 cases of severe PE (sPE), with another 50 normal pregnant women as controls. LncRNA MIR193BHG expression in serum was detected by RT-qPCR. The correlation between MIR193BHG expression and clinical indicators was determined using Pearson analysis. The downstream microRNAs (miRNAs) and genes of MIR193BHG were predicted and verified through the database and dual-luciferase assay. Expressions of miR-345-3p and SASH1 in serum of PE patients were detected using RT-qPCR.
LncRNA MIR193BHG was upregulated in the serum of PE patients, and MIR193BHG expression in mPE patients was lower than that in sPE patients. MIR193BHG expression was positively correlated with systolic and diastolic blood pressure, and urine protein. miR-345-3p was poorly expressed and SASH was highly expressed in serum of PE patients. There existed a binding relationship between MIR193BHG and miR-345-3p or between miR-345-3p and SASH.
LncRNA MIR193BHG was upregulated in the serum of PE patients. Moreover, MIR193BHG might play a role in PE by competitively binding to SASH1 with miR-345-3p.
子痫前期（PE）是晚期妊娠的一种显著并发症。长链非编码RNA（LncRNAs）是PE中的关键生物调节因子。本研究调查了lncRNA MIR193BHG在PE中的表达及其临床意义。
收集116例PE患者的血清样本，其中包括62例轻度PE（mPE）患者和54例重度PE（sPE）患者，另选取50例正常孕妇作为对照。采用RT-qPCR检测血清中lncRNA MIR193BHG的表达。使用Pearson分析确定MIR193BHG表达与临床指标之间的相关性。通过数据库和双荧光素酶测定法预测并验证MIR193BHG的下游微小RNA（miRNAs）和基因。采用RT-qPCR检测PE患者血清中miR-345-3p和SASH1的表达。
lncRNA MIR193BHG在PE患者血清中上调，mPE患者中MIR193BHG的表达低于sPE患者。MIR193BHG表达与收缩压、舒张压及尿蛋白呈正相关。PE患者血清中miR-345-3p表达较低，SASH表达较高。MIR193BHG与miR-345-3p之间或miR-345-3p与SASH之间存在结合关系。
lncRNA MIR193BHG在PE患者血清中上调。此外，MIR193BHG可能通过与miR-345-3p竞争性结合SASH1在PE中发挥作用。
