微小RNA-425-5p通过靶向膜联蛋白A2调节骨质疏松症。
MicroRNA-425-5p modulates osteoporosis by targeting annexin A2.
作者信息
Chen Guanghua, Huang Guizhi, Lin Han, Wu Xinyou, Tan Xiaoyan, Chen Zhoutao
机构信息
Department of Orthopedics, Affiliated Hospital of Guangdong Medical University, NO.57, Renmin Dadao, Xiashan District, Zhanjiang, 524001, Guangdong Province, China.
出版信息
Immun Ageing. 2021 Dec 8;18(1):45. doi: 10.1186/s12979-021-00256-7.
BACKGROUND
Studies have shown that the decrease of osteogenic differentiation of bone marrow mesenchymal stem cells (MSC) is an important mechanism of osteoporosis. The object of this study was to explore the role and mechanism of microRNA miR-425-5p in the differentiation of MSC.
METHODS
The expression of miR-425-5p in MSC was detected by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Cell proliferation, cell cycle and apoptosis were detected by CCK-8 colorimetry and flow cytometry. The expression of TNF were detected by ELISA.
RESULTS
Our data show that MiR-425-5p could modulate TNF-induced cell apoptosis, proliferation, and differentiation. ANXA2 is also the target of miR-425-5p and ANXA2 was involved in TNF-induced MSC cell apoptosis, proliferation, and differentiation. In addition, MiR-425-5p enhanced osteoporosis in mice.
CONCLUSION
MiR-425-5p might serve as a potential therapeutic target for the treatment of osteoporosis.
背景
研究表明，骨髓间充质干细胞（MSC）成骨分化能力下降是骨质疏松症的重要发病机制。本研究旨在探讨微小RNA miR-425-5p在MSC分化中的作用及机制。
方法
采用定量逆转录聚合酶链反应（qRT-PCR）检测miR-425-5p在MSC中的表达。通过CCK-8比色法和流式细胞术检测细胞增殖、细胞周期和细胞凋亡。采用ELISA法检测TNF的表达。
结果
我们的数据表明，MiR-425-5p可调节TNF诱导的细胞凋亡、增殖和分化。膜联蛋白A2（ANXA2）也是miR-425-5p的靶标，且ANXA2参与了TNF诱导的MSC细胞凋亡、增殖和分化。此外，MiR-425-5p加重了小鼠的骨质疏松症。
结论
MiR-425-5p可能是治疗骨质疏松症的潜在治疗靶点。
Zotero 插件