Department of Thoracic Surgery, Vanderbilt University Medical Center, Nashville, Tennessee.
Division of Hepatobiliary Surgery and Liver Transplantation, Department of Surgery.
J Am Assoc Lab Anim Sci. 2022 Jan 1;61(1):101-104. doi: 10.30802/AALAS-JAALAS-21-000074. Epub 2021 Dec 8.
Swine () are commonly used large animal subjects for the study of disease and preclinical therapies. Organ machine perfusion is a therapy that has gained momentum as a research platform for the study of ex vivo organ preservation and therapeutics. However, complex perfusion circuits and research protocols often require large volumes of blood as perfusate. Here, we report a technique for increasing terminal blood yield during swine organ and blood procurement; our method involves acute normovolemic hemodilution and exsanguination via the femoral artery. We collected a total of 47 ± 4 mL/kg of blood and 4.3 ± 0.6 g/kg of hemoglobin, representing 73% ± 6% of the estimated blood volume and 64% ± 8% of the total estimated intravascular hemoglobin ( = 4). Neither pH, lactate, nor pO2 levels changed significantly during blood procurement. Acute normovolemic hemodilution is an effective method for increasing RBC and hemoglobin yield during blood procurement in swine.
猪通常被用作研究疾病和临床前治疗的大型动物实验对象。器官机器灌注是一种治疗方法，作为离体器官保存和治疗的研究平台，已逐渐普及。然而，复杂的灌注回路和研究方案通常需要大量血液作为灌注液。在这里，我们报告了一种在猪器官和血液采集过程中增加终末血液产量的技术；我们的方法涉及急性等容血液稀释和经股动脉放血。我们共采集了 47 ± 4 mL/kg 的血液和 4.3 ± 0.6 g/kg 的血红蛋白，分别代表估计血容量的 73% ± 6%和总血管内血红蛋白的 64% ± 8%（= 4）。在血液采集过程中，pH 值、乳酸水平和 pO2 水平均无明显变化。急性等容血液稀释是一种在猪血液采集过程中增加红细胞和血红蛋白产量的有效方法。
