Medical Parasitology Department, Faculty of Medicine, Cairo University, Cairo, Egypt; Medical Parasitology Department, Faculty of Medicine, Armed Forces College of Medicine, Cairo, Egypt.
Medical Microbiology and Immunology Department, Faculty of Medicine, Fayoum University, Fayoum, Egypt.
Vet Parasitol Reg Stud Reports. 2021 Dec;26:100635. doi: 10.1016/j.vprsr.2021.100635. Epub 2021 Sep 9.
To investigate the immuno-histological evidences in viable and non-viable hydatid cysts obtained from naturally infected camels.
A cohort study (February 2018-December 2019), a total of 15 hydatidosis-infected camels from slaughter houses in Cairo were involved. Specimens were investigated for parasite viability, liver histological changes, IL-17A cytokine immunohistochemical expressions in the adventitial layer, and the anti-nuclear antibodies (ANAs) immunofluorescent expression in the metacestode's structures. Real-Time Quantitative -Morphocytometry and SPSS were utilized.
Multi-focal lesions and high viability were found in 60% of the cases. Overall accumulation of collagen associated the parasite establishment that involved infiltrations of mononuclear cells with significantly increased IL-17A expression. Interestingly, the ANAs appeared to have a role in the immune-defense against the metacestode showing different patterns. ANAs production correlated with IL-17A expression and the viability of the parasite.
IL-17A responses in hydatidosis is associated with collagen deposition and ANA production as a sort of anti-parasite immunity in a viability dependent manner.
研究从自然感染的骆驼中获得的活和非活包虫囊肿的免疫组织学证据。
这是一项队列研究（2018 年 2 月至 2019 年 12 月），共涉及来自开罗屠宰场的 15 例包虫病感染骆驼。对寄生虫的生存能力、肝脏组织学变化、外膜层中 IL-17A 细胞因子的免疫组织化学表达以及囊蚴结构中的抗核抗体（ANA）免疫荧光表达进行了研究。使用实时定量形态细胞术和 SPSS 进行分析。
60%的病例发现多灶性病变和高生存能力。与寄生虫定植相关的胶原总体积聚涉及单核细胞浸润，IL-17A 表达显著增加。有趣的是，ANA 似乎在针对包虫蚴的免疫防御中发挥作用，表现出不同的模式。ANA 的产生与 IL-17A 表达和寄生虫的生存能力相关。
包虫病中的 IL-17A 反应与胶原沉积和 ANA 产生相关，是一种依赖于寄生虫生存能力的抗寄生虫免疫。
