[OK-432诱导细胞毒性因子进入腹腔液（PCF）。II. PCF的物理化学和免疫学特性]

[Induction of a cytotoxic factor into the peritoneal fluid (PCF) by OK-432. II. Physicochemical and immunological characteristics of PCF].

作者信息

Nagamuta M, Yamamoto A, Usami H, Sugawara Y, Nishii Y, Watanabe N, Niitsu Y, Urushizaki I

出版信息

Gan To Kagaku Ryoho. 1986 Jul;13(7):2358-62.

Abstract

A cytotoxic factor (PCF = peritoneal cytotoxic factor) was strongly induced by the injection of LPS into the peritoneal fluids of mice which had been previously primed with OK-432. The similarity in biological activity of PCF, TNF and lymphotoxin led us to study the relationships among the three. When incubated with LPS, the macrophages from the mice primed with OK-432 induced PCF, whereas the lymphocytes did not. These results indicate that PCF is different from lymphotoxin. PCF appears to be identical to Tumor Necrosis Factor (TNF) in the serum for the following reasons: The two factors are similar in their modes of cytotoxic action in vitro. Both factors have a tumor-necrotizing effect when injected into tumor-bearing mice. Both are produced from macrophages. They are similar in their physicochemical characteristics. The cytotoxic activity of PCF was totally abolished by anti-TNF serum.

摘要

通过向预先用OK-432致敏的小鼠腹腔注射脂多糖（LPS），可强烈诱导出一种细胞毒性因子（PCF = 腹腔细胞毒性因子）。PCF、肿瘤坏死因子（TNF）和淋巴毒素在生物学活性上的相似性促使我们研究这三者之间的关系。用LPS孵育时，预先用OK-432致敏的小鼠的巨噬细胞可诱导产生PCF，而淋巴细胞则不能。这些结果表明PCF与淋巴毒素不同。由于以下原因，PCF似乎与血清中的肿瘤坏死因子（TNF）相同：这两种因子在体外的细胞毒性作用方式相似。将这两种因子注射到荷瘤小鼠体内时都具有肿瘤坏死作用。两者均由巨噬细胞产生。它们在物理化学特性上相似。PCF的细胞毒性活性被抗TNF血清完全消除。