对OK-432诱导小鼠腹腔液中产生的细胞毒性因子的特性研究。

Characterization of a cytotoxic factor induced in mouse peritoneal fluid by OK-432.

作者信息

Nagamuta M, Yamamoto A, Usami H, Sugawara Y, Watanabe N, Niitsu Y, Urushizaki I

出版信息

Immunol Lett. 1986 Aug;13(1-2):101-5. doi: 10.1016/0165-2478(86)90134-3.

Abstract

A cytotoxic factor (peritoneal cytotoxic factor, PCF) was strongly induced by the injection of LPS into the peritoneal fluids of mice which had been previously primed with OK-432. In order to clarify characteristics of PCF, physicochemical and immunological studies were conducted. When incubated with LPS, the macrophages from mice primed with OK-432 induced PCF whereas the lymphocytes did not. These results indicate that PCF is different from lymphotoxin. PCF appears to be quite similar to tumor necrosis factor (TNF) in the serum for the following reasons: The two factors are similar in the mode of cytotoxic action in vitro; both factors have a tumor necrotizing effect when injected into tumor bearing mice; both are produced from macrophages; they are similar in physicochemical characteristics; and the cytotoxic activity of PCF is totally abolished by anti-TNF serum.

摘要

通过向预先用OK-432致敏的小鼠腹腔注射脂多糖（LPS），可强烈诱导出一种细胞毒性因子（腹腔细胞毒性因子，PCF）。为阐明PCF的特性，进行了理化和免疫学研究。用LPS孵育时，预先用OK-432致敏的小鼠巨噬细胞可诱导产生PCF，而淋巴细胞则不能。这些结果表明PCF不同于淋巴毒素。PCF在血清中似乎与肿瘤坏死因子（TNF）非常相似，原因如下：这两种因子在体外细胞毒性作用方式上相似；注入荷瘤小鼠体内时，二者均具有肿瘤坏死作用；二者均由巨噬细胞产生；它们在理化特性上相似；并且PCF的细胞毒性活性被抗TNF血清完全消除。