广西壮族人群中MYO9B基因多态性与炎症性肠病的相关性
The Correlation Between MYO9B Gene Polymorphism and Inflammatory Bowel Disease in the Guangxi Zhuang Population.
作者信息
Zeng Rui-Zhi, Lv Xiao-Dan, Liu Geng-Feng, Gu Guang-Li, Li Shi-Quan, Chen Lan, Fan Jun-Hua, Liang Zhao-Liang, Wang Hui-Qin, Lu Fei, Zhan Ling-Ling, Lv Xiao-Ping
机构信息
Department of Gastroenterology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi Zhuang Autonomous Region, People's Republic of China.
Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi Zhuang Autonomous Region, People's Republic of China.
出版信息
Int J Gen Med. 2021 Dec 1;14:9163-9172. doi: 10.2147/IJGM.S338142. eCollection 2021.
OBJECTIVE
To analyze the correlation between site rs962917 of the MYO9B gene and inflammatory bowel disease (IBD) in the Guangxi Zhuang nationality population.
METHODS
The intestinal mucosa tissue of 153 IBD subjects (Han and Zhuang patients only) in the Guangxi Zhuang autonomous region comprised the case group, and the intestinal mucosa tissue of 155 healthy subjects (Han and Zhuang patients only) in the same region represented the control group. Deoxyribonucleic acid was extracted from the intestinal mucosa tissue of each experimental group, and the MYO9B gene-target fragment containing the single nucleotide polymorphism (SNP) site rs962917 was designed. Finally, polymerase chain reaction products were obtained by amplification, analyzed, and compared using the sequencing results.
RESULTS
The results indicated that the genotype frequency of the MYO9B SNP site rs962917 between Crohn's disease (CD) and control groups of Zhuang and Han participants differed significantly (P < 0.05). Furthermore, the genotype frequency of MYO9B site rs962917 differed significantly between the Zhuang and Han population groups (P < 0.05).
CONCLUSION
Site rs962917 of the MYO9B gene is related to CD susceptibility and incidence among the Guangxi Zhuang population.
目的
分析广西壮族人群中MYO9B基因rs962917位点与炎症性肠病（IBD）的相关性。
方法
以广西壮族自治区153例IBD患者（仅汉族和壮族患者）的肠黏膜组织为病例组，同一地区155例健康受试者（仅汉族和壮族患者）的肠黏膜组织为对照组。从各实验组的肠黏膜组织中提取脱氧核糖核酸，设计包含单核苷酸多态性（SNP）位点rs962917的MYO9B基因靶向片段。最后，通过扩增获得聚合酶链反应产物，利用测序结果进行分析和比较。
结果
结果表明，壮族和汉族参与者的克罗恩病（CD）组与对照组之间MYO9B SNP位点rs962917的基因型频率差异显著（P < 0.05）。此外，壮族和汉族人群组之间MYO9B位点rs962917的基因型频率差异显著（P < 0.05）。
结论
MYO9B基因的rs962917位点与广西壮族人群的CD易感性和发病率有关。
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