Bly J E, Cuchens M A, Clem L W
Immunology. 1986 Jul;58(3):523-6.
Low (non-permissive) temperatures inhibit the proliferation responses of channel catfish T cells to stimulation with Con A. The study reported here was undertaken to ascertain if failure to bind Con A at non-permissive temperatures could explain the observed suppression at such temperatures. The influence of temperature on the binding of fluorescein-labelled succinyl Con A to channel catfish T and B cells was studied by cytofluorography. The results indicated that Con A bound equally well at both mitogenically permissive (22 degrees, 27 degrees and 32 degrees) and non-permissive (12 degrees and 17 degrees) temperatures. Hence, temperature effects on mitogen-binding to cell surface receptors cannot readily explain the observed suppression of channel catfish T-cell proliferative responses at non-permissive temperatures.
低温（非允许温度）会抑制斑点叉尾鮰T细胞对刀豆蛋白A刺激的增殖反应。本文所报道的研究旨在确定在非允许温度下不能结合刀豆蛋白A是否可以解释在此类温度下观察到的抑制现象。通过细胞荧光成像技术研究了温度对荧光素标记的琥珀酰刀豆蛋白A与斑点叉尾鮰T细胞和B细胞结合的影响。结果表明，刀豆蛋白A在促有丝分裂允许温度（22℃、27℃和32℃）和非允许温度（12℃和17℃）下的结合效果相同。因此，温度对有丝分裂原与细胞表面受体结合的影响不能轻易解释在非允许温度下观察到的斑点叉尾鮰T细胞增殖反应的抑制现象。
