Miller N W, Sizemore R C, Clem L W
J Immunol. 1985 May;134(5):2884-8.
Three functionally distinct leukocyte subpopulations were isolated from the peripheral blood of channel catfish. Surface immunoglobulin-positive (sIg+) and sIg- lymphocytes were isolated by an indirect "planning" procedure employing monoclonal antibodies specific for channel catfish Ig. A third population composed of macrophages was isolated by adherence to baby hamster kidney cell microexudate-coated surfaces. Functional features of these three cell types were established by assessing their role(s) in primary in vitro anti-hapten PFC responses to known murine thymus-dependent (TD) and thymus-independent (TI) antigens. The results indicated that anti-hapten PFC responses to a TI antigen required the presence of sIg+ lymphocytes and macrophages. In contrast, all three cell types were required for responses to TD antigens. Furthermore, the results of studies involving the depletion of antigen-reactive lymphocytes demonstrated that both hapten-specific sIg+ cells and carrier-specific sIg- cells were required for anti-hapten responses to TD antigens. These studies provide direct evidence that catfish have separable B cells (sIg+ lymphocytes), T helper cells (sIg- lymphocytes), and accessory cells (macrophages) quite similar to those seen in higher animals.
从斑点叉尾鮰的外周血中分离出了三种功能不同的白细胞亚群。通过使用针对斑点叉尾鮰免疫球蛋白的单克隆抗体的间接“淘选”程序，分离出了表面免疫球蛋白阳性（sIg+）和sIg-淋巴细胞。通过黏附于幼仓鼠肾细胞微渗出物包被的表面，分离出了由巨噬细胞组成的第三种细胞群。通过评估这三种细胞类型在体外对已知的鼠胸腺依赖性（TD）和胸腺非依赖性（TI）抗原的初次抗半抗原PFC反应中的作用，确定了它们的功能特征。结果表明，对TI抗原的抗半抗原PFC反应需要sIg+淋巴细胞和巨噬细胞的存在。相比之下，对TD抗原的反应则需要所有三种细胞类型。此外，涉及消耗抗原反应性淋巴细胞的研究结果表明，对TD抗原的抗半抗原反应需要半抗原特异性sIg+细胞和载体特异性sIg-细胞。这些研究提供了直接证据，表明鲶鱼具有与高等动物中所见的非常相似的可分离的B细胞（sIg+淋巴细胞）、T辅助细胞（sIg-淋巴细胞）和辅助细胞（巨噬细胞）。
