Deschavanne P J, Debieu D, Fertil B, Malaise E P
Int J Radiat Biol Relat Stud Phys Chem Med. 1986 Aug;50(2):279-93. doi: 10.1080/09553008614550661.
A statistical analysis of the radiosensitivity of 204 different survival curves of nontransformed human fibroblast cell strains of different genetic origins was made using three criteria: the multi-target one-hit model (characterized by parameters n and D0), the surviving fraction for a 2 Gy dose (S2) and the mean inactivation dose (D). D is found to be the best parameter for characterization of anomalous radiosensitivity linked to a genetic disorder and for discrimination between groups of cell strains of differing radiosensitivity. Its use allows the description of a range of 'normal' radiosensitivity for control fibroblasts and the classification of the various genetic disorders as a function of their mean radiosensitivity expressed in terms of D. Nine groups of cell strains appear to exhibit radiosensitivity which differs significantly from that of the controls: seven groups are hypersensitive (ataxia-telangiectasia homozygotes and heterozygotes, Cockayne's syndrome, Gardner's syndrome, 5-oxoprolinuria homozygotes and heterozygotes, Fanconi's anaemia) and two groups are more radioresistant (fibroblasts from retinoblastoma patients and from individuals with chromosome 13 anomalies). Since the coupled parameter n and D0 failed to discriminate between the radiosensitivity of the different genetic groups, we recommend the use of D to make an intercomparison of intrinsic radiosensitivity of nontransformed human fibroblasts.
使用三个标准对204种不同遗传起源的未转化人类成纤维细胞系的存活曲线的放射敏感性进行了统计分析：多靶单击模型（由参数n和D0表征）、2 Gy剂量时的存活分数（S2）和平均失活剂量（D）。发现D是表征与遗传疾病相关的异常放射敏感性以及区分不同放射敏感性细胞系组的最佳参数。使用它可以描述对照成纤维细胞的一系列“正常”放射敏感性，并根据以D表示的平均放射敏感性对各种遗传疾病进行分类。九组细胞系似乎表现出与对照有显著差异的放射敏感性：七组为超敏（共济失调毛细血管扩张症纯合子和杂合子、科凯恩综合征、加德纳综合征、5-氧脯氨酸尿症纯合子和杂合子、范可尼贫血），两组更具放射抗性（视网膜母细胞瘤患者和成13号染色体异常个体的成纤维细胞）。由于耦合参数n和D0未能区分不同遗传组的放射敏感性，我们建议使用D对未转化人类成纤维细胞的内在放射敏感性进行相互比较。
