Current understanding and optimization strategies for efficient lignin-enzyme interaction: A review.

From energy perspective, with abundant polysaccharides (45-85%), the renewable lignocellulosic is recognized as the 2nd generation feedstock for bioethanol and bio-based products production. Enzymatic hydrolysis is a critical pathway to yield fermentable monosaccharides from pretreated substrates of lignocellulose. Nevertheless, the lignin presence in lignocellulosic substrates leads to the low substrate enzymatic digestibility ascribed to the nonproductive adsorption. It has been reported that the water-soluble lignin (low molecular weight, sulfonated/sulfomethylated and graft polymer) enhance the rate of enzymatic digestibility, however, the catalytic mechanism of lignin-enzyme interaction remains elusive. In this review, optimization strategies for enzymatic hydrolysis based on the lignin structural modification, enzyme engineering, and different additives are critically reviewed. Lignin-enzyme interaction mechanism is also discussed (lignin and various cellulases). In addition, the mathematical models and simulation of lignin, cellulose and enzyme aims for promoting an integrated biomass-conversion process for sustainable production of value-added biofuels.