Li Zhen, Li Lu, Sun Haoyu, Wang Wenjuan, Yang Yuying, Qi Zhengliang, Liu Xinli
Key Laboratory of Shandong Microbial Engineering, College of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong, PR China; State Key Laboratory of Bio-based Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong, PR China.
Key Laboratory of Shandong Microbial Engineering, College of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong, PR China.
Bioresour Technol. 2022 Feb;345:126495. doi: 10.1016/j.biortech.2021.126495. Epub 2021 Dec 6.
NO-N and NH-N are two prevalent nitrogenous pollutants in aquaculture wastewater posing a significant health risk to aquatic animals. R. azotoformans ATCC17025 can rapidly denitrify to remove NO-N, assimilating NH-N. The study investigated the influence of ammonia assimilation on bacterial denitrification. Results revealed that low concentration of NH-N (≤0.3 mM) accelerated denitrification, whereas high concentration inhibited it. RT-qPCR indicated that the inhibition of NO reduction under high concentration of NH-N was the primary cause of denitrification depression, whereas low concentration of NH-N enhanced the synthesis of practically all enzymes involved in denitrification. Finally, nitrogen-rich aquaculture effluent was effectively treated in lab-scale using a semi-continuous operation that provided an appropriate NH-N concentration for denitrification. This semi-continuous operation treated wastewater 2 times faster than the batch operation and the content of nitrogen decreased to effluent standard. The study can provide guidance for nitrogen removal of aquaculture wastewater with bioaugmentation.
亚硝酸盐氮和氨氮是水产养殖废水中两种常见的含氮污染物，对水生动物构成重大健康风险。固氮螺菌ATCC17025能够快速进行反硝化作用以去除亚硝酸盐氮，并同化氨氮。该研究调查了氨同化作用对细菌反硝化的影响。结果表明，低浓度的氨氮（≤0.3 mM）会加速反硝化作用，而高浓度则会抑制反硝化作用。逆转录定量聚合酶链反应表明，高浓度氨氮下亚硝酸盐还原的抑制是反硝化作用受抑制的主要原因，而低浓度氨氮则增强了几乎所有参与反硝化作用的酶的合成。最后，在实验室规模上采用半连续操作有效地处理了富含氮的水产养殖废水，该操作可为反硝化作用提供合适的氨氮浓度。这种半连续操作处理废水的速度比间歇操作快两倍，并且氮含量降至排放标准。该研究可为生物强化去除水产养殖废水中的氮提供指导。
