Xie Huifang, Ma Xin, Lin Wenbin, Dong Shiting, Liu Qiang, Chen Yi, Gao Qunyu
Carbohydrate Laboratory, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
School of Computer Science and Technology, Tiangong University, Tianjin 300387, China.
Polymers (Basel). 2021 Nov 30;13(23):4187. doi: 10.3390/polym13234187.
In the current study, linear dextrin (LD) was prepared using waxy potato starch debranched with pullulanase, which has attracted immense interest in the food, pharmaceutical, and cosmetic industries as a versatile ingredient. Various LDs were separated on the basis of their differential solubility in aqueous/ethanol solutions of different volumetric ratios. Three LD products-LD Fabrications with 40% ethanol (F-40); LD Fabrications with 50% ethanol (F-50); and LD Fabrications with 60%, 70%, and 80% ethanol (F-M)-were obtained with an average degree of polymerization (DP) values of 31.44, 21.84, and 16.10, respectively. The results of Fourier transform infrared spectroscopy (FT-IR) analysis revealed that the reaction mainly involved hydrogen bonding and a hydrophobic interaction between LD and insulin in the process of inclusion complex formation. X-ray diffraction (XRD) results indicated that insulin was encapsulated in LD. The results of circular dichroism (CD) showed that the changes in the secondary structure of insulin were negligible during the release from the inclusion complexes. The order of encapsulation capacity is as follows: the complex composed of F-M and insulin (F-M-INS) > the complex composed of LD and insulin (LD-INS) > the complex composed of F-50 and insulin (F-50-INS) > and the complex composed of F-40 and insulin (F-40-INS). F-M-INS inclusion complexes showed a better effect on reducing the release of insulin in gastric juice and promoting the release of insulin in intestinal juice and blood plasma than LD-INS.
在当前的研究中，线性糊精（LD）是使用经支链淀粉酶脱支的糯马铃薯淀粉制备的，作为一种多功能成分，它在食品、制药和化妆品行业引起了极大的关注。基于它们在不同体积比的水/乙醇溶液中的不同溶解度，分离出了各种LD。获得了三种LD产品：含40%乙醇的LD制剂（F-40）；含50%乙醇的LD制剂（F-50）；以及含60%、70%和80%乙醇的LD制剂（F-M），其平均聚合度（DP）值分别为31.44、21.84和16.10。傅里叶变换红外光谱（FT-IR）分析结果表明，在包合物形成过程中，反应主要涉及LD与胰岛素之间的氢键和疏水相互作用。X射线衍射（XRD）结果表明胰岛素被包裹在LD中。圆二色性（CD）结果表明，胰岛素从包合物中释放过程中二级结构的变化可以忽略不计。包封能力的顺序如下：由F-M和胰岛素组成的复合物（F-M-INS）＞由LD和胰岛素组成的复合物（LD-INS）＞由F-50和胰岛素组成的复合物（F-50-INS）＞由F-40和胰岛素组成的复合物（F-40-INS）。与LD-INS相比，F-M-INS包合物在减少胃液中胰岛素释放以及促进肠液和血浆中胰岛素释放方面表现出更好的效果。
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