State Key Laboratory of Crop Biology, Key Laboratory of Crop Biology and Genetic Improvement of Horticultural Crops in Huanghuai Region, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, China.
Int J Mol Sci. 2021 Nov 29;22(23):12910. doi: 10.3390/ijms222312910.
Hydrogen sulfide (HS) plays a crucial role in regulating chilling tolerance. However, the role of hydrogen peroxide (HO) and auxin in HS-induced signal transduction in the chilling stress response of plants was unclear. In this study, 1.0 mM exogenous HO and 75 μM indole-3-acetic acid (IAA) significantly improved the chilling tolerance of cucumber seedlings, as demonstrated by the mild plant chilling injury symptoms, lower chilling injury index (CI), electrolyte leakage (EL), and malondialdehyde content (MDA) as well as higher levels of photosynthesis and cold-responsive genes under chilling stress. IAA-induced chilling tolerance was weakened by N, N'-dimethylthiourea (DMTU, a scavenger of HO), but the polar transport inhibitor of IAA (1-naphthylphthalamic acid, NPA) did not affect HO-induced mitigation of chilling stress. IAA significantly enhanced endogenous HO synthesis, but HO had minimal effects on endogenous IAA content in cucumber seedlings. In addition, the HO scavenger DMTU, inhibitor of HO synthesis (diphenyleneiodonium chloride, DPI), and IAA polar transport inhibitor NPA reduced HS-induced chilling tolerance. Sodium hydrosulfide (NaHS) increased HO and IAA levels, flavin monooxygenase (FMO) activity, and respiratory burst oxidase homolog () and FMO-like protein () mRNA levels in cucumber seedlings. DMTU, DPI, and NPA diminished NaHS-induced HO production, but DMTU and DPI did not affect IAA levels induced by NaHS during chilling stress. Taken together, the present data indicate that HO as a downstream signal of IAA mediates HS-induced chilling tolerance in cucumber seedlings.
硫化氢 (HS) 在调节冷胁迫耐受方面起着至关重要的作用。然而，在植物冷胁迫反应中，过氧化氢 (HO) 和生长素在 HS 诱导的信号转导中的作用尚不清楚。在这项研究中，1.0 mM 外源 HO 和 75 μM 吲哚-3-乙酸 (IAA) 显著提高了黄瓜幼苗的冷胁迫耐受能力，表现在植株冷胁迫损伤症状较轻、冷胁迫损伤指数 (CI)、电解质渗漏 (EL) 和丙二醛含量 (MDA) 较低，以及在冷胁迫下光合作用和冷响应基因水平较高。IAA 诱导的冷胁迫耐受性被 HO 的清除剂 N, N'-二甲基硫脲 (DMTU) 削弱，但 IAA 的极性运输抑制剂 1-萘基邻氨甲酰苯甲酸 (NPA) 对 HO 缓解冷胁迫的作用没有影响。IAA 显著增强了内源 HO 的合成，但 HO 对内源 IAA 含量在黄瓜幼苗中影响很小。此外，HO 清除剂 DMTU、HO 合成抑制剂 (二苯基碘氯化物，DPI) 和 IAA 极性运输抑制剂 NPA 降低了 HS 诱导的冷胁迫耐受性。硫氢化钠 (NaHS) 增加了 HO 和 IAA 的水平、黄素单加氧酶 (FMO) 活性以及呼吸爆发氧化酶同源物 () 和 FMO 样蛋白 () 的 mRNA 水平在黄瓜幼苗中。DMTU、DPI 和 NPA 减少了 NaHS 诱导的 HO 产生，但 DMTU 和 DPI 不影响 NaHS 在冷胁迫下诱导的 IAA 水平。总之，这些数据表明 HO 作为 IAA 的下游信号，介导了 HS 诱导的黄瓜幼苗冷胁迫耐受。
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