Sun Nan, Li Can, Jiang Xiangning, Gai Ying
College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
Plants (Basel). 2023 Jan 4;12(2):227. doi: 10.3390/plants12020227.
ATP-binding cassette transporters (ABC transporters) play crucial physiological roles in plants, such as being involved in the growth and development of organs, nutrient acquisition, response to biotic and abiotic stress, disease resistance, and the interaction of the plant with its environment. The ABCG subfamily of proteins are involved in the process of plant vegetative organ development. In contrast, the functions of the ABCG36 and ABCG40 transporters have received considerably less attention. Here, we investigated changes in the transcriptomic data of the stem tissue of transgenic tobacco () with and () overexpression, and compared them with those of the wild type (WT). Compared with the WT, we identified 1120 and 318 differentially expressed genes (DEGs) in the and groups, respectively. We then annotated the function of the DEGs against the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. The results showed enrichment in cell wall biogenesis and hormone signal transduction functional classes in transgenic tobacco. In transgenic tobacco, the enrichment was involved in metabolic and biosynthetic processes, mainly those related to environmental adaptation. In addition, among these DEGs, many auxin-related genes were significantly upregulated in the group, and we found key genes involved in environmental adaptation in the group, including an encoding resistance protein and a WRKY transcription factor. These results suggest that and play important roles in plant development and environmental adaptation. may promote plant organ growth and development by increasing auxin transport, whereas may inhibit the expression of to improve the resistance of transgenic tobacco. Our results are beneficial to researchers pursuing further study of the functions of ABCG36 and ABCG40.
ATP结合盒转运蛋白（ABC转运蛋白）在植物中发挥着关键的生理作用，例如参与器官的生长发育、养分获取、对生物和非生物胁迫的响应、抗病性以及植物与环境的相互作用。ABCG蛋白亚家族参与植物营养器官的发育过程。相比之下，ABCG36和ABCG40转运蛋白的功能受到的关注要少得多。在这里，我们研究了过表达ABCG36和ABCG40的转基因烟草茎组织转录组数据的变化，并将其与野生型（WT）进行比较。与WT相比，我们分别在ABCG36和ABCG40组中鉴定出1120个和318个差异表达基因（DEG）。然后，我们根据基因本体论（GO）和京都基因与基因组百科全书（KEGG）数据库对DEG的功能进行注释。结果表明，转基因ABCG36烟草在细胞壁生物合成和激素信号转导功能类别中富集。在转基因ABCG40烟草中，富集涉及代谢和生物合成过程，主要是那些与环境适应相关的过程。此外，在这些DEG中，许多生长素相关基因在ABCG36组中显著上调，并且我们在ABCG40组中发现了参与环境适应的关键基因，包括一个编码抗性蛋白的基因和一个WRKY转录因子。这些结果表明，ABCG36和ABCG40在植物发育和环境适应中发挥重要作用。ABCG36可能通过增加生长素运输促进植物器官的生长发育，而ABCG40可能抑制ABCG36的表达以提高转基因烟草的抗性。我们的结果有助于研究人员进一步研究ABCG36和ABCG40的功能。
