迷迭香酸和柠檬酸钠通过抑制铁摄取对 种具有协同抑菌作用。
Rosmarinic Acid and Sodium Citrate Have a Synergistic Bacteriostatic Effect against Species by Inhibiting Iron Uptake.
机构信息
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Agricultural Bioinformatics Research Unit, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
出版信息
Int J Mol Sci. 2021 Dec 1;22(23):13010. doi: 10.3390/ijms222313010.
BACKGROUND
New strategies are needed to combat multidrug-resistant bacteria. The restriction of iron uptake by bacteria is a promising way to inhibit their growth. We aimed to suppress the growth of bacterial species by inhibiting their ferric ion-binding protein (FbpA) using food components.
METHODS
Twenty spices were selected for the screening of FbpA inhibitors. The candidate was applied to antibacterial tests, and the mechanism was further studied.
RESULTS
An active compound, rosmarinic acid (RA), was screened out. RA binds competitively and more tightly than Fe to VmFbpA, the FbpA from , with apparent values of 8 μM vs. 17 μM. Moreover, RA can inhibit the growth of to one-third of the control at 1000 μM. Interestingly, sodium citrate (SC) enhances the growth inhibition effect of RA, although SC only does not inhibit the growth. The combination of RA/SC completely inhibits the growth of not only at 100/100 μM but also the vibriosis-causative pathogens and , at 100/100 and 1000/100 μM, respectively. However, RA/SC does not affect the growth of .
CONCLUSIONS
RA/SC is a potential bacteriostatic agent against species while causing little damage to indigenous gastrointestinal bacteria.
背景
需要新的策略来对抗多药耐药菌。限制细菌摄取铁是抑制其生长的一种有前途的方法。我们旨在通过使用食物成分抑制细菌的亚铁结合蛋白 (FbpA) 来抑制细菌的生长。
方法
选择了 20 种香料来筛选 FbpA 抑制剂。将候选物应用于抗菌测试，并进一步研究其机制。
结果
筛选出一种活性化合物迷迭香酸 (RA)。RA 与 VmFbpA（来自 ）的 Fe 竞争结合且结合更紧密，表观 值为 8 μM 对 17 μM。此外，RA 可将 的生长抑制至对照的三分之一，浓度为 1000 μM。有趣的是，柠檬酸钠 (SC) 增强了 RA 的生长抑制作用，尽管 SC 本身并不抑制生长。RA/SC 的组合完全抑制了 100/100 μM 浓度下不仅 的生长，还抑制了 100/100 和 1000/100 μM 浓度下的弧菌病病原体 和 的生长，但 RA/SC 对 没有影响。
结论
RA/SC 是一种针对 种细菌的潜在抑菌剂，同时对肠道内固有细菌的生长几乎没有影响。
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